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TMEM199 Polyclonal antibody

TMEM199 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 25489-1-AP

Print datasheet

Synonyms

C17orf32, TMEM199, transmembrane protein 199

验证数据展示

  • WB analysis of NIH/3T3 using 25489-1-AP

    WB analysis of NIH/3T3 using 25489-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25489-1-AP (TMEM199 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 25489-1-AP

    IHC staining of human breast cancer using 25489-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25489-1-AP (TMEM199 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human breast cancer using 25489-1-AP

    IHC staining of human breast cancer using 25489-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25489-1-AP (TMEM199 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inNIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25489-1-AP is TMEM199 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Tested Reactivity human, mouse
Immunogen TMEM199 fusion protein Ag22009 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transmembrane protein 199
Synonyms C17orf32, TMEM199, transmembrane protein 199
Calculated Molecular Weight 208 aa, 23 kDa
Observed Molecular Weight 18-23 kDa
GenBank Accession NumberBC033113
Gene Symbol TMEM199
Gene ID (NCBI) 147007
RRIDAB_2880101
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N511
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM199, also named as C17orf32, is a component of the complex sense antisense architecture (CSAGA).

实验方案

Product Specific Protocols
WB protocol for TMEM199 antibody 25489-1-APDownload protocol
IHC protocol for TMEM199 antibody 25489-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols