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验证数据展示

  • WB analysis of NIH/3T3 using 25489-1-AP

    WB analysis of NIH/3T3 using 25489-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25489-1-AP (TMEM199 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 25489-1-AP

    IHC staining of human breast cancer using 25489-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25489-1-AP (TMEM199 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human breast cancer using 25489-1-AP

    IHC staining of human breast cancer using 25489-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25489-1-AP (TMEM199 Antibody) at dilution of 1:50 (under 40x lens).

TMEM199 Polyclonal antibody

TMEM199 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25489-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

C17orf32, TMEM199, transmembrane protein 199

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

25489-1-AP targets TMEM199 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag22009

Product name: Recombinant human TMEM199 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-208 aa of BC033113

Sequence: MASSLLAGERLVRALGPGGELEPERLPRKLRAELEAALGKKHKGGDSSSGPQRLVSFRLIRDLHQHLRERDSKLYLHELLEGSEIYLPEVVKPPRNPELVARLEKIKIQLANEEYKRITRNVTCQDTRHGGTLSDLGKQVRSLKALVITIFNFIVTVVAAFVCTYLGSQYIFTEMASRVLAALIVASVVGLAELYVMVRAMEGELGEL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 199
别名 C17orf32, TMEM199, transmembrane protein 199
计算分子量 208 aa, 23 kDa
观测分子量 18-23 kDa
GenBank蛋白编号BC033113
基因名称 TMEM199
Gene ID (NCBI) 147007
RRIDAB_2880101
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N511
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM199, also named as C17orf32, is a component of the complex sense antisense architecture (CSAGA).

实验方案

Product Specific Protocols
WB protocol for TMEM199 antibody 25489-1-APDownload protocol
IHC protocol for TMEM199 antibody 25489-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oncogene

USP33 facilitates the ovarian cancer progression via deubiquitinating and stabilizing CBX2

Authors - Jiming Chen
View Article