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验证数据展示

  • IHC staining of human liver using 21355-1-AP

    IHC staining of human liver using 21355-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21355-1-AP (TMEM195 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 21355-1-AP

    IHC staining of human liver using 21355-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21355-1-AP (TMEM195 antibody) at dilution of 1:200 (under 40x lens).

  • WB analysis of HepG2 using 21355-1-AP

    WB analysis of HepG2 using 21355-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21355-1-AP (TMEM195 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

TMEM195 Polyclonal antibody

TMEM195 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21355-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Alkylglycerol monooxygenase, TMEM195, transmembrane protein 195

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, L02 cells, MCF-7 cells, mouse liver tissue
Positive IHC detected inhuman liver tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

21355-1-AP targets TMEM195 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原 TMEM195 fusion protein Ag15774 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 195
别名 Alkylglycerol monooxygenase, TMEM195, transmembrane protein 195
计算分子量 445 aa, 52 kDa
观测分子量 52 kDa
GenBank蛋白编号BC108676
基因名称 TMEM195
Gene ID (NCBI) 392636
RRIDAB_2878845
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6ZNB7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TMEM195 antibody 21355-1-APDownload protocol
IHC protocol for TMEM195 antibody 21355-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Infect Dis

Biochemical Characterization of AGMO Variants Implicated in Relapses in Visceral Leishmaniasis.

Authors - Katrin Watschinger
View Article
humanWB

Hum Genet

Biallelic variants in AGMO with diminished enzyme activity are associated with a neurodevelopmental disorder.

Authors - Volkan Okur
View Article