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验证数据展示

  • IHC staining of mouse brain using 28031-1-AP

    IHC staining of mouse brain using 28031-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28031-1-AP (TMEM184B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 28031-1-AP

    IHC staining of mouse brain using 28031-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28031-1-AP (TMEM184B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TMEM184B Polyclonal antibody

TMEM184B Polyclonal Antibody for IHC, ELISA
Cat No. 28031-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

IHC, ELISA

别名

C22orf5, DKFZp586A1024, DKFZp686F07174, DKFZp686N1150, FM08, HS5O6A, TMEM184B, transmembrane protein 184B

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28031-1-AP targets TMEM184B in IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag27704

Product name: Recombinant human TMEM184B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 318-407 aa of BC015489

Sequence: VYADKRLDAQGRCAPMKSISSSLKETMNPHDIVQDAIHNFSPAYQQYTQQSTLEPGPTWRGGAHGLSRSHSLSGARDNEKTLLLSSDDEF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 184B
别名 C22orf5, DKFZp586A1024, DKFZp686F07174, DKFZp686N1150, FM08, HS5O6A, TMEM184B, transmembrane protein 184B
GenBank蛋白编号BC015489
基因名称 TMEM184B
Gene ID (NCBI) 25829
RRIDAB_3086023
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y519
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transmembrane protein 184B (TMEM184B) is a seven-pass transmembrane protein highly expressed in the nervous system and is thought to play a role in neuronal excitability, synaptic structure, and expression of key developmental and adult pathways involved in neuronal differentiation (PMID: 27122027; 37730546). TMEM184B may activate the MAP kinase signaling pathway (PMID: 12761501). TMEM184B has also been reported to be necessary for interleukin-31-induced itch, promoting adult somatosensation through developmental Wnt signaling and promotion of proper pruriceptive gene expression (PMID: 34629389).

实验方案

Product Specific Protocols
IHC protocol for TMEM184B antibody 28031-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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