• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of A431 using 55411-1-AP

    WB analysis of A431 using 55411-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 55411-1-AP (TMEM181 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 55411-1-AP

    IHC staining of mouse heart using 55411-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 55411-1-AP (TMEM181 Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with TMEM181 antibody included

TMEM181 Polyclonal antibody

TMEM181 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 55411-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

GPR178, KIAA1423, TMEM181, transmembrane protein 181

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inA431 cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TMEM181 Ready-To-Use IHC Kit. TMEM181 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55411-1-AP targets TMEM181 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 181
别名 GPR178, KIAA1423, TMEM181, transmembrane protein 181
计算分子量 69 kDa
观测分子量 75 kDa
GenBank蛋白编号NM_020823
基因名称 TMEM181
Gene ID (NCBI) 57583
RRIDAB_11182284
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P2C4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM181, also named as GPR178, KIAA1423, is a membrane protein. It mediates action of cytolethal distending toxins (CDT), which are secreted by many pathogenic bacteria. Expression level of TMEM181 is rate-limiting for intoxication. This antibody is specific to TMEM181.

实验方案

Product Specific Protocols
WB protocol for TMEM181 antibody 55411-1-APDownload protocol
IHC protocol for TMEM181 antibody 55411-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols