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验证数据展示

  • WB analysis of mouse liver using 24799-1-AP

    WB analysis of mouse liver using 24799-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 24799-1-AP (TMEM179 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 24799-1-AP

    IHC staining of human breast cancer using 24799-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24799-1-AP (TMEM179 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 24799-1-AP

    IHC staining of human breast cancer using 24799-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24799-1-AP (TMEM179 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

TMEM179 Polyclonal antibody

TMEM179 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24799-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA and More (1)

别名

C14orf90, TMEM179, TMEM179A, transmembrane protein 179, Transmembrane protein 179A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24799-1-AP targets TMEM179 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原

CatNo: Ag20502

Product name: Recombinant human TMEM179 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 115-197 aa of BC148844

Sequence: AFVVFLVFIASTIVSVGFTMWCDTITEKGTVPHSCEELQDIDLELGVDNSAFYDQFAIAQVGGSGQEGRLAMLGGGHLLLDIC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 179
别名 C14orf90, TMEM179, TMEM179A, transmembrane protein 179, Transmembrane protein 179A
计算分子量 233 aa, 26 kDa
观测分子量 32 kDa
GenBank蛋白编号BC148844
基因名称 TMEM179
Gene ID (NCBI) 388021
RRIDAB_2879732
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6ZVK1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TMEM179 antibody 24799-1-APDownload protocol
IHC protocol for TMEM179 antibody 24799-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Ecotoxicol Environ Saf

NAC antagonizes arsenic-induced neurotoxicity through TMEM179 by inhibiting oxidative stress in Oli-neu cells.

Authors - Zhixin He
  • KD Validated
View Article
humanWB

PLoS One

Construction of ceRNA network to identify the lncRNA and mRNA related to non-small cell lung cancer.

Authors - Kui Xiao
View Article