• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of fetal human brain using 20378-1-AP

    WB analysis of fetal human brain using 20378-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 20378-1-AP (TMEM176A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 20378-1-AP

    IHC staining of mouse brain using 20378-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20378-1-AP (TMEM176A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 20378-1-AP

    IHC staining of mouse brain using 20378-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20378-1-AP (TMEM176A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 20378-1-AP

    IF Staining of HEK-293 using 20378-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using TMEM176A antibody (20378-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

TMEM176A Polyclonal antibody

TMEM176A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20378-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

GS188, HCA112, Hepatocellular carcinoma-associated antigen 112, Transmembrane protein 176A

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected infetal human brain tissue
Positive IHC detected inmouse brain tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20378-1-AP targets TMEM176A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag14194

Product name: Recombinant human TMEM176A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 123-203 aa of BC008303

Sequence: LAAFSTAIAALKLWNEDFRYGYSYYNSACRISSSSDWNTPAPTQSPEEVRRLHLCTSFMDMLKALFRTLQAMLLGVWILLL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 176A
别名 GS188, HCA112, Hepatocellular carcinoma-associated antigen 112, Transmembrane protein 176A
计算分子量 235 aa, 26 kDa
观测分子量 28 kDa
GenBank蛋白编号BC008303
基因名称 TMEM176A
Gene ID (NCBI) 55365
RRIDAB_10697661
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96HP8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for TMEM176A antibody 20378-1-APDownload protocol
IHC protocol for TMEM176A antibody 20378-1-APDownload protocol
WB protocol for TMEM176A antibody 20378-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...