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验证数据展示

  • WB analysis using 84554-1-RR (same clone as 84554-1-PBS)

    WB analysis using 84554-1-RR (same clone as 84554-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84554-1-RR (TMEM175 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84554-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 84554-1-RR (same clone as 84554-1-PBS)

    WB analysis of HepG2 using 84554-1-RR (same clone as 84554-1-PBS)

    WB result of TMEM175 antibody (84554-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM175 transfected HepG2 cells. This data was developed using the same antibody clone with 84554-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84554-1-RR

    Affinity and Kinetic Characterization of 84554-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84554-1-RR against Human TMEM175 were performed. The affinity constant is 5.17 nM.

  • Featured Product
  • KD/KO验证

TMEM175 Recombinant monoclonal antibody, PBS Only

TMEM175 Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 84554-1-PBS

产品说明书

CloneNo. 241567B9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

241567B9, hTMEM175, Transmembrane protein 175

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84554-1-PBS targets TMEM175 in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag13890

Product name: Recombinant human TMEM175 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 222-313 aa of BC005158

Sequence: YVSKVTGWCRDRLLGHREPSAHPVEVFSFDLHEPLSKERVEAFSDGVYAIVATLLILDICEDNVPDPKDVKERFSGSLVAALSATGPRFLAY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transmembrane protein 175
别名 241567B9, hTMEM175, Transmembrane protein 175
计算分子量 504 aa, 56 kDa
观测分子量56 kDa
GenBank蛋白编号BC005158
基因名称 TMEM175
Gene ID (NCBI) 84286
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9BSA9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TMEM175 has two repeats of 6-transmembrane-spanning segments and has no GYG K+ channel sequence signature-containing, pore-forming P loop. Lysosomes lacking TMEM175 exhibit no K+conductance, have a markedly depolarized ΔΨ and little sensitivity to changes in [K+], and have compromised luminal pH stability and abnormal fusion with autophagosomes during autophagy. TMEM175 comprises a K+ channel that underlies the molecular mechanism of lysosomal K+ permeability. It has two isoforms with MW 41-45 kDa and 54-60 kDa. For optimal WB detection with this antibody, we recommend avoiding boiling the sample after lysis.

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