验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, SH-SY5Y cells, HepG2 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19925-1-AP targets TMEM175 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, monkey |
| 免疫原 |
CatNo: Ag13890 Product name: Recombinant human TMEM175 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 222-313 aa of BC005158 Sequence: YVSKVTGWCRDRLLGHREPSAHPVEVFSFDLHEPLSKERVEAFSDGVYAIVATLLILDICEDNVPDPKDVKERFSGSLVAALSATGPRFLAY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transmembrane protein 175 |
| 别名 | Transmembrane protein 175, hTMEM175 |
| 计算分子量 | 504 aa, 56 kDa |
| 观测分子量 | 41-45 kDa, 54-60 kDa |
| GenBank蛋白编号 | BC005158 |
| 基因名称 | TMEM175 |
| Gene ID (NCBI) | 84286 |
| RRID | AB_10666166 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BSA9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TMEM175 has two repeats of 6-transmembrane-spanning segments and has no GYG K+ channel sequence signature-containing, pore-forming P loop. Lysosomes lacking TMEM175 exhibit no K+conductance, have a markedly depolarized ΔΨ and little sensitivity to changes in [K+], and have compromised luminal pH stability and abnormal fusion with autophagosomes during autophagy. TMEM175 comprises a K+ channel that underlies the molecular mechanism of lysosomal K+ permeability. It has two isoforms with MW 41-45 kDa and 54-60 kDa. For optimal WB detection with this antibody, we recommend avoiding boiling the sample after lysis.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for TMEM175 antibody 19925-1-AP | Download protocol |
| WB protocol for TMEM175 antibody 19925-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature A growth-factor-activated lysosomal K+ channel regulates Parkinson's pathology.
| ||
Ann Neurol Genetic, Structural, and Functional Evidence Link TMEM175 to Synucleinopathies. | ||
Proc Natl Acad Sci U S A TMEM175 deficiency impairs lysosomal and mitochondrial function and increases α-synuclein aggregation.
| ||
Mol Med Modulation of anti-cardiac fibrosis immune responses by changing M2 macrophages into M1 macrophages | ||
Hum Mol Genet Mitochondrial clearance and maturation of autophagosomes are compromised in LRRK2 G2019S familial Parkinson's disease patient fibroblasts. |
