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验证数据展示

  • IHC staining of human lung cancer using 33212-1-AP

    IHC staining of human lung cancer using 33212-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 33212-1-AP (TMEM16A/DOG1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TMEM16A/DOG1 antibody included

TMEM16A/DOG1 Polyclonal antibody

TMEM16A/DOG1 Polyclonal Antibody for IHC, ELISA
Cat No. 33212-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, ELISA

别名

ANO1, Anoctamin 1, Anoctamin-1, Discovered on gastrointestinal stromal tumors protein 1, DOG1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TMEM16A/DOG1 Ready-To-Use IHC Kit. TMEM16A/DOG1 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

33212-1-AP targets TMEM16A/DOG1 in IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human
免疫原 TMEM16A/DOG1 fusion protein Ag37099 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 anoctamin 1, calcium activated chloride channel
别名 ANO1, Anoctamin 1, Anoctamin-1, Discovered on gastrointestinal stromal tumors protein 1, DOG1
计算分子量114kDa，986aa
GenBank蛋白编号NM_018043.5
基因名称 TMEM16A
Gene ID (NCBI) 55107
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ5XXA6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ANO1 also named as DOG1, ORAOV2, TAOS2 and TMEM16A, acts as a calcium-activated chloride channel. It is required for normal tracheal development. ANO1 (or DOG1) is used as an aid in the identification and diagnosis of gastrointestinal stromal tumors (GIST) within the context of an antibody panel, the patient's clinical history, and other diagnostic tests evaluated by a qualified pathologist.

实验方案

Product Specific Protocols
IHC protocol for TMEM16A/DOG1 antibody 33212-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols