×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis of Jurkat using 20485-1-AP (same clone as 20485-1-PBS)

    WB analysis of Jurkat using 20485-1-AP (same clone as 20485-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20485-1-AP (TMEM165 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20485-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 20485-1-AP (same clone as 20485-1-PBS)

    IP experiment of HeLa using 20485-1-AP (same clone as 20485-1-PBS)

    IP result of anti-TMEM165 (IP:20485-1-AP, 4ug; Detection:20485-1-AP 1:1000) with HeLa cells lysate 1750 ug. This data was developed using the same antibody clone with 20485-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 20485-1-AP (same clone as 20485-1-PBS)

    IF Staining of HeLa using 20485-1-AP (same clone as 20485-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TMEM165 antibody (20485-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 20485-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 20485-1-AP (same clone as 20485-1-PBS)

    IF Staining of HeLa using 20485-1-AP (same clone as 20485-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TMEM165 antibody (20485-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594-Phalloidin (red). This data was developed using the same antibody clone with 20485-1-PBS in a different storage buffer formulation.

TMEM165 Polyclonal antibody, PBS Only

TMEM165 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 20485-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, Indirect ELISA

别名

Putative divalent cation/proton antiporter TMEM165, TMPT27, TPARL, Transmembrane protein 165, Transmembrane protein PT27

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

20485-1-PBS targets TMEM165 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag14362

Product name: Recombinant human TMEM165 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 61-177 aa of BC003545

Sequence: VSTVLFAIFGIRMLREGLKMSPDEGQEELEEVQAELKKKDEEFQRTKLLNGPGDVETGTSITVPQKKWLHFISPIFVQALTLTFLAEWGDRSQLTTIVLAAREDPYGVAVGGTVGHC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 165
别名 Putative divalent cation/proton antiporter TMEM165, TMPT27, TPARL, Transmembrane protein 165, Transmembrane protein PT27
计算分子量 324 aa, 35 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC003545
基因名称 TMEM165
Gene ID (NCBI) 55858
RRIDAB_10695184
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HC07
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TMEM165 is a putative divalent cation:proton antiporter that exchanges calcium or manganese ions for protons across the Golgi membrane. It mediates the reversible transport of calcium or manganese to the Golgi lumen driven by the proton gradient and possibly the membrane potential generated by V-ATPase, and provides calcium or manganese cofactors to resident Golgi enzymes and contributes to the maintenance of an acidic luminal Golgi pH required for proper functioning of the secretory pathway (PMID:22683087, 23569283, 27008884, 32047108). TMEM165 has two isoforms, with molecular weights of 28 kDa and 35 kDa respectively. It is also subject to ubiquitination modification, which may cause an increase in its molecular weight.

||
New chat

Able™

正在加载，请稍候...