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验证数据展示

  • IHC staining of mouse skeletal muscle using 31625-1-AP

    IHC staining of mouse skeletal muscle using 31625-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 31625-1-AP (TMEM164 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 31625-1-AP

    IF Staining of U2OS using 31625-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using TMEM164 antibody (31625-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

  • IHC staining of mouse skeletal muscle using 31625-1-AP

    IHC staining of mouse skeletal muscle using 31625-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 31625-1-AP (TMEM164 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TMEM164 Polyclonal antibody

TMEM164 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 31625-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, ELISA

别名

bB360B22.3, transmembrane protein 164

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31625-1-AP targets TMEM164 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag34317

Product name: Recombinant human TMEM164 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-38 aa of BC085605

Sequence: MSRYSYQSLLDWLYGGVDPSFAGNGGPDCAAFLSWQQR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 164
别名 bB360B22.3, transmembrane protein 164
GenBank蛋白编号BC085605
基因名称 TMEM164
Gene ID (NCBI) 84187
RRIDAB_3742456
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ5U3C3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM164 (Transmembrane Protein 164) is a membrane protein with significant roles in various biological processes, including lipid metabolism, neuroprotection, and cancer regulation. In non-small cell lung cancer (NSCLC), TMEM164 promotes ferroptosis by mediating autophagy, leading to the degradation of ferritin, GPX4, and lipid droplets, which facilitates iron accumulation and lipid peroxidation (PMID: 39798196). In neurodegenerative diseases, TMEM164 overexpression via gene therapy shows promise in reducing neurotoxic reactive astrocyte activation and alleviating disease symptoms (PMID: 37679556).

实验方案

Product Specific Protocols
IF protocol for TMEM164 antibody 31625-1-APDownload protocol
IHC protocol for TMEM164 antibody 31625-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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