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验证数据展示

  • IHC staining of mouse brain using 24898-1-AP

    IHC staining of mouse brain using 24898-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24898-1-AP (TMEM161A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 24898-1-AP

    IHC staining of mouse brain using 24898-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24898-1-AP (TMEM161A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 24898-1-AP

    WB analysis using 24898-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24898-1-AP (TMEM161A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

TMEM161A Polyclonal antibody

TMEM161A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24898-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

UNQ582/PRO1152, Transmembrane protein 161A, AROS-29, AROS 29, Adaptive response to oxidative stress protein 29

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inmouse brain tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24898-1-AP targets TMEM161A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TMEM161A fusion protein Ag18974 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 161A
别名 UNQ582/PRO1152, Transmembrane protein 161A, AROS-29, AROS 29, Adaptive response to oxidative stress protein 29
计算分子量 479 aa, 54 kDa
观测分子量45 kDa
GenBank蛋白编号BC005210
基因名称 TMEM161A
Gene ID (NCBI) 54929
RRIDAB_2879785
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NX61
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM161A also named as transmembrane protein 161A is a 479 amino acid protein which belongs to TMEM161 family. The molecular weight of TMEM161A is 56 kDa or 42 kDa. TMEM161A is a multi-pass membrane protein and localizes to the cell membrane. It may be involved in the negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage and cellular response to oxidative stress.

实验方案

Product Specific Protocols
WB protocol for TMEM161A antibody 24898-1-APDownload protocol
IHC protocol for TMEM161A antibody 24898-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols