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验证数据展示

  • WB analysis using 31917-1-AP

    WB analysis using 31917-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31917-1-AP (TMEM143 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 31917-1-AP

    IHC staining of human placenta using 31917-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31917-1-AP (TMEM143 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 31917-1-AP

    IHC staining of human placenta using 31917-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31917-1-AP (TMEM143 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TMEM143 Polyclonal antibody

TMEM143 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31917-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

UNQ5922/PRO19813, Transmembrane protein 143

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHH cells, HepG2 cells, Jurkat cells, Ramos cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31917-1-AP targets TMEM143 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 TMEM143 fusion protein Ag36277 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 143
别名 UNQ5922/PRO19813, Transmembrane protein 143
计算分子量52 kDa
观测分子量45-52 kDa
GenBank蛋白编号BC016919
基因名称 TMEM143
Gene ID (NCBI) 55260
RRIDAB_3670142
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ96AN5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transmembrane protein 143 (TMEM143) is a transmembrane protein and a member of the TMEM (Transmembrane) protein family, which is located in mitochondria (PMID: 11256614). It is known that members of the TMEM family play an important role in the development and metastasis of cancer, especially in conveying information between the extracellular environment and cytoplasmic proteins (PMID: 31454669).

实验方案

Product Specific Protocols
WB protocol for TMEM143 antibody 31917-1-APDownload protocol
IHC protocol for TMEM143 antibody 31917-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols