验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31327-1-AP targets TMEM135 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag34057 Product name: Recombinant human TMEM135 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 352-458 aa of BC051462 Sequence: YLASKLVETMYFKGIEAGKVPYFPHADTIIYSISTAICFQAAVMEVQTLRPSYWKFLLRLTKGKFAVMNRKVLDVFGTGASKHFQDFIPRLDPRYTTVTPELPTEFS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transmembrane protein 135 |
| 别名 | transmembrane protein 135, PMP52 |
| 计算分子量 | 458 aa, 52 kDa |
| 观测分子量 | 52 kDa |
| GenBank蛋白编号 | BC051462 |
| 基因名称 | TMEM135 |
| Gene ID (NCBI) | 65084 |
| RRID | AB_3669944 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q86UB9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TMEM135, also known as PMP52, contains 3 N-terminal transmembrane domains and 4 C-terminal transmembrane domains. TMEM135 is up-regulated in bone marrow-derived mesenchymal stem cells during differentiation into adipocytes compared to osteoblasts(PMID: 18637193).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TMEM135 antibody 31327-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
