验证数据展示
经过测试的应用
| Positive WB detected in | HaCaT cells, fetal human brain tissue, MCF-7 cells, SH-SY5Y cells, U-87 MG cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 2 publications below |
产品信息
25301-1-AP targets TMEM132A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag18142 Product name: Recombinant human TMEM132A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 636-850 aa of BC114569 Sequence: VLELRVQPVMGISLTLSRGTAHPGEVTATCWAQSALPAPKQEVALSLWLSFSDHTVAPAELYDRRDLGLSVSAEEPGAILPAEEQGAQLGVVVSGAGAEGLPLHVALHPPEPCRRGRHRVPLASGTAWLGLPPASTPAPALPSSPAWSPPATEATMGGKRQVAGSVGGNTGVRGKFERAEEEARKEETEAREEEEEEEEEMVPAPQHVTELELGM 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transmembrane protein 132A |
| 别名 | GBP, HSPA5 binding protein 1, HSPA5-binding protein 1, HSPA5BP1, KIAA1583 |
| 计算分子量 | 1023 aa, 110 kDa |
| 观测分子量 | 100-140 kDa |
| GenBank蛋白编号 | BC114569 |
| 基因名称 | TMEM132A |
| Gene ID (NCBI) | 54972 |
| RRID | AB_2880018 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q24JP5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TMEM132A antibody 25301-1-AP | Download protocol |
| IHC protocol for TMEM132A antibody 25301-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol TMEM132A, a Novel Wnt Signaling Pathway Regulator Through Wntless (WLS) Interaction.
| ||
Development TMEM132A ensures mouse caudal neural tube closure and regulates integrin-based mesodermal migration
|