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验证数据展示

  • IP experiment of HeLa using 23142-1-AP

    IP experiment of HeLa using 23142-1-AP

    IP result of anti-TMEM127 (IP:23142-1-AP, 3ug; Detection:23142-1-AP 1:500) with HeLa cells lysate 3000ug.

  • IHC staining of human stomach cancer using 23142-1-AP

    IHC staining of human stomach cancer using 23142-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23142-1-AP (TMEM127 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TMEM127 antibody included
  • IHC staining of human stomach cancer using 23142-1-AP

    IHC staining of human stomach cancer using 23142-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23142-1-AP (TMEM127 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TMEM127 antibody included
  • IF Staining of NIH/3T3 using 23142-1-AP

    IF Staining of NIH/3T3 using 23142-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using TMEM127 antibody (23142-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

TMEM127 Polyclonal antibody

TMEM127 Polyclonal Antibody for IHC, IF/ICC, IP, ELISA
Cat No. 23142-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, IP, ELISA and More (1)

别名

Transmembrane protein 127

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IP detected inHeLa cells
Positive IHC detected inhuman stomach cancer tissue, human heart tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy TMEM127 Ready-To-Use IHC Kit. TMEM127 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

23142-1-AP targets TMEM127 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原

CatNo: Ag19472

Product name: Recombinant human TMEM127 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 139-238 aa of BC039892

Sequence: QCATVIGFSYWASELILAQQQQHKKYHGSQVYVTFAVSFYLVAGAGGASILATAANLLRHYPTEEEEQALELLSEMEENEPYPAEYEVINQFQPPPAYTP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 127
别名 Transmembrane protein 127
计算分子量 238 aa, 26 kDa
观测分子量 26 kDa
GenBank蛋白编号BC039892
基因名称 TMEM127
Gene ID (NCBI) 55654
RRIDAB_2879217
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDO75204
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for TMEM127 antibody 23142-1-APDownload protocol
IF protocol for TMEM127 antibody 23142-1-APDownload protocol
IP protocol for TMEM127 antibody 23142-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

Elife

A cleaved METTL3 potentiates the METTL3-WTAP interaction and breast cancer progression

Authors - Chaojun Yan
View Article
humanWB

Tissue Cell

Exosomes from adipose-derived stem cells overexpressing microRNA-671-3p promote fat graft angiogenesis and adipogenic differentiation

Authors - Xiaoyan Hao
View Article