• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of mouse liver using CL647-27585

    IF Staining of mouse liver using CL647-27585

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using CoraLite® Plus 647 TMEM119 antibody (CL647-27585) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HEK-293 using CL647-27585

    FC experiment of HEK-293 using CL647-27585

    1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated TMEM119 Polyclonal antibody (CL647-27585)(red), or 0.4 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

CoraLite® Plus 647-conjugated TMEM119 Polyclonal antibody

TMEM119 Polyclonal Antibody for IF-P, FC (Intra)
Cat No. CL647-27585

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF-P, FC (Intra)

别名

OBIF, Osteoblast induction factor, transmembrane protein 119, UNQ731/PRO1415

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 647
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF-P detected inmouse liver tissue
Positive FC (Intra) detected inHEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 27585-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-27585 targets TMEM119 in IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF-P, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag26269

Product name: Recombinant human TMEM119 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 119-218 aa of NM_181724

Sequence: MRQKQKASAYYPSSFPKKKYVDQSDRAGGPRAFSEVPDRAPDSRPEEALDSSRQLQADILAATQNLKSPTRAALGGGDGARMVEGRGAEEEEKGSQEGDQE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 119
别名 OBIF, Osteoblast induction factor, transmembrane protein 119, UNQ731/PRO1415
计算分子量 29 kDa
观测分子量 45 kDa
GenBank蛋白编号NM_181724
基因名称 TMEM119
Gene ID (NCBI) 338773
RRIDAB_3673571
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ4V9L6
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM119 immunohistochemistry might provide a useful tool for investigating the biology and pathology of human microglia(PMID: 26250788). Microglia can be detected clearly using Catalog#27585-1-AP. The predicted MW of TMEM119 is 29 kDa. Catalog#27585-1-AP recognizes 45-50 kDa band may due to glycosylation..

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 TMEM119 antibody CL647-27585Download protocol
IF protocol for CL Plus 647 TMEM119 antibody CL647-27585Download protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...