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验证数据展示

  • IHC staining of Human brain cancer（Astrocitoma） using 83448-9-RR

    IHC staining of Human brain cancer（Astrocitoma） using 83448-9-RR

    Immunohistochemical analysis of paraffin-embedded Human brain cancer(Astrocitoma) slide using 83448-9-RR (TMEM119 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 83448-9-RR

    IHC staining of mouse brain using 83448-9-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83448-9-RR (TMEM119 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 83448-9-RR

    IF Staining of mouse brain using 83448-9-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TMEM119 antibody (83448-9-RR, Clone: 251557E2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 83448-9-RR

    IF Staining of mouse brain using 83448-9-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TMEM119 antibody (83448-9-RR, Clone: 251557E2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite® Plus 647 IBA1 antibody (CL647-81728, Clone: 4D5, Magenta), GFAP antibody (60190-1-Ig, Clone: 4B2E10, orange). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 83448-9-RR

    IF Staining of mouse brain using 83448-9-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TMEM119 antibody (83448-9-RR, Clone: 251557E2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite® Plus 647 IBA1 antibody (CL647-81728, Clone: 4D5, Magenta), GFAP antibody (60190-1-Ig, Clone: 4B2E10, orange). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity of 83448-9-RR

    Affinity of 83448-9-RR

    Biolayer interferometry (BLI) kinetic assays of 83448-9-RR against Human TMEM119 were performed. The affinity constant is 3.12 nM.

TMEM119 Recombinant monoclonal antibody

TMEM119 Uni-rAb® Recombinant Antibody for IHC, IF-P, ELISA
Cat No. 83448-9-RR

产品说明书

CloneNo. 251557E2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF-P, ELISA

别名

OBIF, Osteoblast induction factor, transmembrane protein 119, UNQ731/PRO1415

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 647
规格价格库存

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经过测试的应用

Positive IHC detected inHuman brain cancer（Astrocitoma）, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83448-9-RR targets TMEM119 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Eg1823

Product name: Recombinant human TMEM119 protein

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 26-96 aa of NM_181724

Sequence: RSVPLKATFLEDVAGSGEAEGSSASSPSLPPPWTPALSPTSMGPQPITLGGPSPPTNFLDGIVDFFRQYVM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transmembrane protein 119
别名 OBIF, Osteoblast induction factor, transmembrane protein 119, UNQ731/PRO1415
计算分子量29 kDa
GenBank蛋白编号NM_181724
基因名称 TMEM119
Gene ID (NCBI) 338773
RRIDAB_3743479
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ4V9L6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transmembrane protein 119 (TMEM119), was characterized to be a specific microglia marker by Bennet et al. in 2016, although its function in microglia remains unknown. It is considered to be a marker expressed by microglia under homeostatic conditions based on transcriptomic findings . Under pathological conditions, the immune-specialized brain microenvironment contains both resident microglia and bone marrow-derived myeloid cells recruited from peripheral circulation. Recently, transmembrane protein 119 (TMEM119) has been reported as a marker for resident microglia which is not expressed by bone marrow-derived myeloid cells. (PMID: 35247551, PMID: 38308250, PMID: 40373772)

实验方案

Product Specific Protocols
IF protocol for TMEM119 antibody 83448-9-RRDownload protocol
IHC protocol for TMEM119 antibody 83448-9-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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