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验证数据展示

  • IHC staining of mouse brain using 27585-1-AP (same clone as 27585-1-PBS)

    IHC staining of mouse brain using 27585-1-AP (same clone as 27585-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27585-1-AP (TMEM119 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27585-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 27585-1-AP (same clone as 27585-1-PBS)

    IF Staining of mouse brain using 27585-1-AP (same clone as 27585-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TMEM119 antibody (27585-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27585-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 27585-1-AP (same clone as 27585-1-PBS)

    FC experiment of HEK-293 using 27585-1-AP (same clone as 27585-1-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug TMEM119 Polyclonal antibody (27585-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 27585-1-PBS in a different storage buffer formulation.

TMEM119 Polyclonal antibody, PBS Only

TMEM119 Polyclonal Antibody for IHC, IF-P, FC (Intra), ELISA
Cat No. 27585-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF-P, FC (Intra), ELISA

别名

OBIF, Osteoblast induction factor, transmembrane protein 119, UNQ731/PRO1415

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

27585-1-PBS targets TMEM119 in IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF-P, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag26269

Product name: Recombinant human TMEM119 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 119-218 aa of NM_181724

Sequence: MRQKQKASAYYPSSFPKKKYVDQSDRAGGPRAFSEVPDRAPDSRPEEALDSSRQLQADILAATQNLKSPTRAALGGGDGARMVEGRGAEEEEKGSQEGDQE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 119
别名 OBIF, Osteoblast induction factor, transmembrane protein 119, UNQ731/PRO1415
计算分子量 29 kDa
观测分子量 45 kDa
GenBank蛋白编号NM_181724
基因名称 TMEM119
Gene ID (NCBI) 338773
RRIDAB_2880915
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ4V9L6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TMEM119 immunohistochemistry might provide a useful tool for investigating the biology and pathology of human microglia(PMID: 26250788). Microglia can be detected clearly using Catalog#27585-1-AP.

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