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验证数据展示

  • WB analysis of U2OS using 25536-1-AP

    WB analysis of U2OS using 25536-1-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 25536-1-AP (TMEM115 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 25536-1-AP

    IHC staining of human ovary cancer using 25536-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25536-1-AP (TMEM115 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 25536-1-AP

    IHC staining of human ovary cancer using 25536-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25536-1-AP (TMEM115 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 25536-1-AP

    WB analysis using 25536-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25536-1-AP (TMEM115 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

TMEM115 Polyclonal antibody

TMEM115 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25536-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Transmembrane protein 115, Placental protein 6, PL6, LUCA11.2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inU2OS cells, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inhuman ovary cancer tissue, human intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25536-1-AP targets TMEM115 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TMEM115 fusion protein Ag22258 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 115
别名 Transmembrane protein 115, Placental protein 6, PL6, LUCA11.2
计算分子量 351 aa, 38 kDa
观测分子量35-38 kDa
GenBank蛋白编号BC017367
基因名称 TMEM115
Gene ID (NCBI) 11070
RRIDAB_3669482
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ12893
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM115 also known as PL6, located on chromosome 3p21.3, was originally thought to function as a tumor suppressor. TMEM115 is an integral membrane protein of the Golgi complex involved in retrograde transport.

实验方案

Product Specific Protocols
WB protocol for TMEM115 antibody 25536-1-APDownload protocol
IHC protocol for TMEM115 antibody 25536-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols