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验证数据展示

  • WB analysis using 31438-1-AP

    WB analysis using 31438-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31438-1-AP (TMEM109 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • WB analysis using 31438-1-AP

    WB analysis using 31438-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31438-1-AP (TMEM109 antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse skeletal muscle using 31438-1-AP

    IF Staining of mouse skeletal muscle using 31438-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using TMEM109 antibody (31438-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse skeletal muscle using 31438-1-AP

    IF Staining of mouse skeletal muscle using 31438-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using TMEM109 antibody (31438-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse pancreas using 31438-1-AP

    IHC staining of mouse pancreas using 31438-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 31438-1-AP (TMEM109 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 31438-1-AP

    IHC staining of mouse skeletal muscle using 31438-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 31438-1-AP (TMEM109 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 31438-1-AP

    IHC staining of mouse skeletal muscle using 31438-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 31438-1-AP (TMEM109 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TMEM109 Polyclonal antibody

TMEM109 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 31438-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

Mg23, Mitsugumin 23, transmembrane protein 109

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse heart tissue, HeLa cells, U-251 cells, mouse skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse skeletal muscle tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31438-1-AP targets TMEM109 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag34866

Product name: Recombinant human TMEM109 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 194-243 aa of BC001309

Sequence: ILYALLSRLTGSRASGAQLEAKVRGLERQVEELRWRQRRAAKGARSVEEE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 109
别名 Mg23, Mitsugumin 23, transmembrane protein 109
计算分子量 26 kDa
观测分子量22 kDa
GenBank蛋白编号BC001309
基因名称 TMEM109
Gene ID (NCBI) 79073
RRIDAB_3669983
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9BVC6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM109, also known as mg23 or mitsugumin-23, is a transmembrane protein localized to the sarcoplasmic/endoplasmic reticulum and nuclear membranes. TMEM109 is a ball-shaped cation channel in the sarcoplasmic reticulum (SR).

实验方案

Product Specific Protocols
IF protocol for TMEM109 antibody 31438-1-APDownload protocol
IHC protocol for TMEM109 antibody 31438-1-APDownload protocol
WB protocol for TMEM109 antibody 31438-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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