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验证数据展示

  • WB analysis using 15177-1-AP

    WB analysis using 15177-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15177-1-AP (Retrolinkin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 15177-1-AP

    IHC staining of mouse brain using 15177-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15177-1-AP (Retrolinkin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Retrolinkin Polyclonal antibody

Retrolinkin Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15177-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

TMEM108, UNQ1875/PRO4318, Transmembrane protein 108, RTLN, KIAA1690

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15177-1-AP targets Retrolinkin in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Retrolinkin fusion protein Ag7076 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 108
别名 TMEM108, UNQ1875/PRO4318, Transmembrane protein 108, RTLN, KIAA1690
计算分子量 60 kDa
观测分子量 55-60 kDa and 65-70 kDa
GenBank蛋白编号BC000568
基因名称 TMEM108
Gene ID (NCBI) 66000
RRIDAB_2204598
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6UXF1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Retrolinkin, also named as TMEM108, KIAA1690 and RTLN, is a membrane protein which plays an important role in retrograde axonal transport. It's highly enriched in neuronal endosomes, binds directly to BPAG1n4. This gene has two isoforms with calculated MW 60 kDa and 50 kDa. With modification, the MW of these two forms will be migrated to 65-70 kDa and 55-60 kDa.

实验方案

Product Specific Protocols
WB protocol for Retrolinkin antibody 15177-1-APDownload protocol
IHC protocol for Retrolinkin antibody 15177-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols