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验证数据展示

  • WB analysis using 84196-2-RR

    WB analysis using 84196-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84196-2-RR (TMEM106B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 84196-2-RR

    IHC staining of mouse cerebellum using 84196-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84196-2-RR (TMEM106B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 84196-2-RR

    IHC staining of mouse cerebellum using 84196-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84196-2-RR (TMEM106B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TMEM106B Recombinant monoclonal antibody

TMEM106B Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 84196-2-RR

产品说明书

CloneNo. 241185F10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

241185F10, Transmembrane protein 106B

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84196-2-RR targets TMEM106B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transmembrane protein 106B
别名 241185F10, Transmembrane protein 106B
计算分子量 31 kDa
观测分子量35 kDa
GenBank蛋白编号BC033901
基因名称 TMEM106B
Gene ID (NCBI) 54664
RRIDAB_3671749
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9NUM4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM106B is a genetic risk factor for frontotemporal lobar degeneration with TDP-43 inclusions (FTLD-TDP). Amyotrophic lateral sclerosis (ALS), like FTLD-TDP, is characterized by pathological TDP-43 inclusions. TMEM106B expression in the brain may be linked to mechanisms of disease in FTLD-TDP and risk alleles confer genetic susceptibility by increasing gene expression. TMEM106B can be showed as 31-55 kDa and 70-90 kDa (Glycosylated or Dimer) form in western blot test. (PMID: 27543298, 22895706, PMID: 23136129).

实验方案

Product Specific Protocols
IHC protocol for TMEM106B antibody 84196-2-RRDownload protocol
WB protocol for TMEM106B antibody 84196-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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