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- KD/KO Validated
TMEM106B Monoclonal antibody
TMEM106B Monoclonal Antibody for IP, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
IP, WB, ELISA and More (2)
Conjugate
Unconjugated
CloneNo.
5D1F8
验证数据展示
经过测试的应用
| Positive WB detected in | U2OS cells, HeLa cells, K-562 cells, LNCaP cells, A549 cells, Jurkat cells |
| Positive IP detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60333-1-Ig targets TMEM106B in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | IP, WB, ELISA |
| Cited Applications | WB, IP, IF, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | TMEM106B fusion protein Ag21448 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | transmembrane protein 106B |
| Synonyms | TMEM106B, transmembrane protein 106B |
| Calculated Molecular Weight | 31 kDa |
| Observed Molecular Weight | ~40 kDa |
| GenBank Accession Number | BC033901 |
| Gene Symbol | TMEM106B |
| Gene ID (NCBI) | 54664 |
| RRID | AB_2881442 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9NUM4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TMEM106B is a genetic risk factor for frontotemporal lobar degeneration with TDP-43 inclusions (FTLD-TDP). Amyotrophic lateral sclerosis (ALS), like FTLD-TDP, is characterized by pathological TDP-43 inclusions. TMEM106B expression in the brain may be linked to mechanisms of disease in FTLD-TDP and risk alleles confer genetic susceptibility by increasing gene expression.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TMEM106B antibody 60333-1-Ig | Download protocol |
| IP protocol for TMEM106B antibody 60333-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Brain C-terminal TMEM106B fragments in human brain correlate with disease-associated TMEM106B haplotypes | ||
Pathol Res Pract TESC acts as a prognostic factor and promotes epithelial-mesenchymal transition progression in esophageal squamous carcinoma | ||
F1000Res The identification of high-performing antibodies for transmembrane protein 106B (TMEM106B) for use in Western blot, immunoprecipitation, and immunofluorescence
| ||
Acta Neuropathol Accumulation of TMEM106B C-terminal fragments in neurodegenerative disease and aging | ||
Front Cell Neurosci Loss of TMEM106B exacerbates C9ALS/FTD DPR pathology by disrupting autophagosome maturation
|
