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验证数据展示

  • WB analysis using 86802-1-RR

    WB analysis using 86802-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86802-1-RR (TMED9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 86802-1-RR

    IF Staining of A549 using 86802-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using TMED9 antibody (86802-1-RR, Clone: 251396A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of MCF-7 using 86802-1-RR

    IF Staining of MCF-7 using 86802-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using TMED9 antibody (86802-1-RR, Clone: 251396A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

TMED9 Recombinant monoclonal antibody

TMED9 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86802-1-RR

产品说明书

CloneNo. 251396A1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Glycoprotein 25L2, GMP25, GP25L2, p24 family protein alpha-2, p24alpha2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inPC-12 cells, MCF-7 cells, OVCAR-3 cells, A2780 cells, HepG2 cells, NIH/3T3 cells
Positive IF/ICC detected inA549 cells, MCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86802-1-RR targets TMED9 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag13813

Product name: Recombinant human TMED9 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-224 aa of BC001123

Sequence: MAVELGVLLVRPRPGTGLGRVMRTLLLVLWLATRGSALYFHIGETEKKCFIEEIPDETMVIGNYRTQLYDKQREEYQPATPGLGMFVEVKDPEDKVILARQYGSEGRFTFTSHTPGEHQICLHSNSTKFSLFAGGMLRVHLDIQVGEHANDYAEIAAKDKLSELQLRVRQLVEQVEQIQKEQNYQRWREERFRQTSESTNQRVLWWSILQTLILVAIGVWQMRH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transmembrane emp24 protein transport domain containing 9
别名 Glycoprotein 25L2, GMP25, GP25L2, p24 family protein alpha-2, p24alpha2
计算分子量 235 aa, 27 kDa
观测分子量 24-27 kDa
GenBank蛋白编号BC001123
基因名称 TMED9
Gene ID (NCBI) 54732
RRIDAB_3745128
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9BVK6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMED9 (also named as GMP25, GP25L2 or p25) is a member of the EMP24/GP25L family. TMED9 probably forms hetero-oligomeric complexes with TMED7 (p27), TMED2 (p24), and TMED10 (p23) (PMID: 10359607). Located in the endoplasmic reticulum and the Golgi, TMED9 appears to be involved in vesicular protein trafficking, mainly in the early secretory pathway (PMID: 9472029; 10359607).

实验方案

Product Specific Protocols
IF protocol for TMED9 antibody 86802-1-RRDownload protocol
WB protocol for TMED9 antibody 86802-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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