• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 14141-1-AP

    WB analysis using 14141-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14141-1-AP (TMED4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse liver using 14141-1-AP

    IP experiment of mouse liver using 14141-1-AP

    IP result of anti-TMED4 (IP:14141-1-AP, 4ug; Detection:14141-1-AP 1:700) with mouse liver tissue lysate 6000 ug.

  • IF Staining of HepG2 using 14141-1-AP

    IF Staining of HepG2 using 14141-1-AP

    Immunofluorescent analysis of HepG2 cells using 14141-1-AP (TMED4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

TMED4 Polyclonal antibody

TMED4 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 14141-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, ELISA

别名

Putative NF-kappa-B-activating protein 156, p24alpha3, p24 family protein alpha-3, GMP25iso, ERS25

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inmouse liver tissue, HepG2 cells, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14141-1-AP targets TMED4 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 TMED4 fusion protein Ag5234 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane emp24 protein transport domain containing 4
别名 Putative NF-kappa-B-activating protein 156, p24alpha3, p24 family protein alpha-3, GMP25iso, ERS25
计算分子量 227 aa, 26 kDa
观测分子量 24-26 kDa
GenBank蛋白编号BC057851
基因名称 TMED4
Gene ID (NCBI) 222068
RRIDAB_10642696
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z7H5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TMED4 antibody 14141-1-APDownload protocol
IF protocol for TMED4 antibody 14141-1-APDownload protocol
IP protocol for TMED4 antibody 14141-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Res

A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis.

Authors - Shulin Li
  • KD Validated
View Article
humanWB

Ann Transl Med

Screening of the novel immune-suppressive biomarkers of TMED family and whether knockdown of TMED2/3/4/9 inhibits cell migration and invasion in breast cancer

Authors - Zheng Fang
  • KD Validated
View Article