验证数据展示
经过测试的应用
| Positive IP detected in | HepG2 cells, HuH-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31488-1-AP targets TMCO3 in IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IP, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag35012 Product name: Recombinant human TMCO3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 23-285 aa of BC068515 Sequence: VEHEEVAQRVIKLHRGRGVAAMQSRQWVRDSCRKLSGLLRQKNAVLNKLKTAIGAVEKDVGLSDEEKLFQVHTFEIFQKELNESENSVFQAVYGLQRALQGDYKDVVNMKESSRQRLEALREAAIKEETEYMELLAAEKHQVEALKNMQHQNQSLSMLDEILEDVRKAADRLEEEIEEHAFDDNKSVKGVNFEAVLRVEEEEANSKQNITKREVEDDLGLSMLIDSQNNQYILTKPRDSTIPRADHHFIKDIVTIGMLSLPCG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transmembrane and coiled-coil domains 3 |
| 别名 | C13orf11, FLJ20623, Putative LAG1-interacting protein, Transmembrane and coiled-coil domain-containing protein 3, UNQ2419/PRO4976 |
| 计算分子量 | 75 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC068515 |
| 基因名称 | TMCO3 |
| Gene ID (NCBI) | 55002 |
| RRID | AB_3742436 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q6UWJ1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TMCO3, also known as transmembrane and coiled-coil domains 3 or C13orf11, is a protein-coding gene with functional annotation of probable Na+/H+ antiporter. It belongs to the monovalent cation:proton antiporter-2 (CPA2) family according to the Transporter Classification Database (TCDB) (PMID: 33170213). Most CPA2 family members have similar functions and are involved in ion transport (K+, Na+, and H+) in bacteria, archaea, insects, and plants. Upregulated TMCO3 could act as an oncogenic mediator and promote sorafenib resistance in HCC, providing a potential therapeutic target for HCC treatment (PMID: 36304514).
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for TMCO3 antibody 31488-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
