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验证数据展示

  • IHC staining of mouse liver using 84499-1-RR

    IHC staining of mouse liver using 84499-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84499-1-RR (TMBIM6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 84499-1-RR

    IHC staining of mouse liver using 84499-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84499-1-RR (TMBIM6 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse liver using 84499-1-RR

    IF Staining of mouse liver using 84499-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using TMBIM6 antibody (84499-1-RR, Clone: 241589D12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84499-1-RR

    Affinity and Kinetic Characterization of 84499-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84499-1-RR against Human TMBIM6 were performed. The affinity constant is 6.46 nM.

TMBIM6 Recombinant monoclonal antibody

TMBIM6 Recombinant Antibody for IHC, IF-P, ELISA
Cat No. 84499-1-RR

产品说明书

CloneNo. 241589D12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF-P, ELISA

别名

TEGT, BI-1, BI1, BI 1, Bax inhibitor 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84499-1-RR targets TMBIM6 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag24780

Product name: Recombinant human TMBIM6 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-86 aa of BC000916

Sequence: MNIFDRKINFDALLKFSHITPSTQQHLKKVYASFALCMFVAAAGAYVHMVTHFIQAGLLSALGSLILMIWLMATPHSHETEQKRLG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transmembrane BAX inhibitor motif containing 6
别名 TEGT, BI-1, BI1, BI 1, Bax inhibitor 1
计算分子量 27 kDa
GenBank蛋白编号BC000916
基因名称 TMBIM6
Gene ID (NCBI) 7009
RRIDAB_3672012
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP55061
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for TMBIM6 antibody 84499-1-RRDownload protocol
IHC protocol for TMBIM6 antibody 84499-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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