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验证数据展示

  • WB analysis using 29520-1-AP

    WB analysis using 29520-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29520-1-AP (TM9SF4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 29520-1-AP

    IHC staining of mouse colon using 29520-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 29520-1-AP (TM9SF4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 29520-1-AP

    IHC staining of human stomach cancer using 29520-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 29520-1-AP (TM9SF4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 29520-1-AP

    IHC staining of human stomach cancer using 29520-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 29520-1-AP (TM9SF4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TM9SF4 Polyclonal antibody

TM9SF4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29520-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IHC, ELISA

别名

dJ836N17.2, KIAA0255, TM9SF4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA375 cells, PC-3 cells, mouse kidney tissue
Positive IHC detected inhuman stomach cancer tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29520-1-AP targets TM9SF4 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse
免疫原 TM9SF4 fusion protein Ag31253 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane 9 superfamily protein member 4
别名 dJ836N17.2, KIAA0255, TM9SF4
计算分子量 642 aa, 75 kDa
观测分子量 70 kDa
GenBank蛋白编号BC021107
基因名称 TM9SF4
Gene ID (NCBI) 9777
RRIDAB_3086139
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92544
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TM9SF4 antibody 29520-1-APDownload protocol
IHC protocol for TM9SF4 antibody 29520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols