验证数据展示
经过测试的应用
Positive WB detected in | A375 cells, A431 cells, HepG2 cells |
Positive IHC detected in | mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
25595-1-AP targets TM9SF4 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IF, IHC |
经测试反应性 | human, mouse |
文献引用反应性 | human, mouse |
免疫原 | TM9SF4 fusion protein Ag22335 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | transmembrane 9 superfamily protein member 4 |
别名 | KIAA0255, TM9SF4 |
计算分子量 | 642 aa, 75 kDa |
观测分子量 | 70 kDa |
GenBank蛋白编号 | BC021107 |
基因名称 | TM9SF4 |
Gene ID (NCBI) | 9777 |
RRID | AB_2880149 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q92544 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for TM9SF4 antibody 25595-1-AP | Download protocol |
IHC protocol for TM9SF4 antibody 25595-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Oncogene Knockdown of TM9SF4 boosts ER stress to trigger cell death of chemoresistant breast cancer cells.
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Stem Cell Res Ther TM9SF4 is a novel regulator in lineage commitment of bone marrow mesenchymal stem cells to either osteoblasts or adipocytes
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J Exp Clin Cancer Res CircCEMIP promotes anoikis-resistance by enhancing protective autophagy in prostate cancer cells. | ||
Cell Mol Gastroenterol Hepatol TM9SF4 Is a Crucial Regulator of Inflammation and ER Stress in Inflammatory Bowel Disease
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Nat Commun TM9SF4 is an F-actin disassembly factor that promotes tumor progression and metastasis
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