验证数据展示
经过测试的应用
| Positive WB detected in | A375 cells, A431 cells, HepG2 cells |
| Positive IHC detected in | mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25595-1-AP targets TM9SF4 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag22335 Product name: Recombinant human TM9SF4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 25-244 aa of BC021107 Sequence: IKAVKLTSSRTQLPYEYYSLPFCQPSKITYKAENLGEVLRGDRIVNTPFQVLMNSEKKCEVLCSQSNKPVTLTVEQSRLVAERITEDYYVHLIADNLPVATRLELYSNRDSDDKKKEKDVQFEHGYRLGFTDVNKIYLHNHLSFILYYHREDMEEDQEHTYRVVRFEVIPQSIRLEDLKADEKSSCTLPEGTNSSPQEIDPTKENQLYFTYSVHWEESDI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transmembrane 9 superfamily protein member 4 |
| 别名 | KIAA0255, TM9SF4 |
| 计算分子量 | 642 aa, 75 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC021107 |
| 基因名称 | TM9SF4 |
| Gene ID (NCBI) | 9777 |
| RRID | AB_2880149 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92544 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for TM9SF4 antibody 25595-1-AP | Download protocol |
| WB protocol for TM9SF4 antibody 25595-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncogene Knockdown of TM9SF4 boosts ER stress to trigger cell death of chemoresistant breast cancer cells.
| ||
Stem Cell Res Ther TM9SF4 is a novel regulator in lineage commitment of bone marrow mesenchymal stem cells to either osteoblasts or adipocytes
| ||
J Exp Clin Cancer Res CircCEMIP promotes anoikis-resistance by enhancing protective autophagy in prostate cancer cells. | ||
Cell Mol Gastroenterol Hepatol TM9SF4 Is a Crucial Regulator of Inflammation and ER Stress in Inflammatory Bowel Disease
| ||
Nat Commun TM9SF4 is an F-actin disassembly factor that promotes tumor progression and metastasis
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