验证数据展示
经过测试的应用
| Positive WB detected in | human lung tissue, BxPC-3 cells, human heart tissue, human kidney tissue |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IHC | See 2 publications below |
产品信息
12033-1-AP targets TM7SF2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag2637 Product name: Recombinant human TM7SF2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 155-418 aa of BC012857 Sequence: APVSALAPGGNSGNPIYDFFLGREVNPRICFFDFKYFCELRPGLIGWVLINLALLMKEAELRGSPSLAMWLVNGFQLLYVGDALWHEEAVLTTMDITHDGFGFMLAFGDMAWVPFTYSLQAQFLLHHPQPLGLPMASVICLINAIGYYIFRGANSQKNTFRKNPSDPRVAGLETISTATGRKLLVSGWWGMVRHPNYLGDLIMALAWSLPCGVSHLLPYFYLLYFTALLVHREARDERQCLQKYGLAWQEYCRRVPYRIMPYIY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transmembrane 7 superfamily member 2 |
| 别名 | C14SR, C-14 sterol reductase, C 14 sterol reductase, Another new gene 1 protein, 3-beta-hydroxysterol Delta |
| 计算分子量 | 418 aa, 46 kDa |
| 观测分子量 | 55-60 kDa |
| GenBank蛋白编号 | BC012857 |
| 基因名称 | TM7SF2 |
| Gene ID (NCBI) | 7108 |
| RRID | AB_2203934 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O76062 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for TM7SF2 antibody 12033-1-AP | Download protocol |
| IHC protocol for TM7SF2 antibody 12033-1-AP | Download protocol |
| WB protocol for TM7SF2 antibody 12033-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Biol Sci METTL16 Promotes Lipid Metabolic Reprogramming and Colorectal Cancer Progression | ||
Phytomedicine Dingxin recipe Ⅲ ameliorates atherosclerosis through stard4-mediated regulation of hepatic lipid metabolism. | ||
J Ethnopharmacol Tylophora yunnanensis extract inhibits cholesterol biosynthesis to suppress triple negative breast cancer | ||
iScience TMEM97 regulates cholesterol biosynthesis and mitochondrial metabolism in gastric carcinoma. | ||
Curr Oncol TM7SF2 as a Potential Biomarker in Colorectal Cancer: Implications for Metastasis | ||
PLoS One Hidden disease susceptibility and sexual dimorphism in the heterozygous knockout of cyp51 from cholesterol synthesis. |
