验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, L02 cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27671-1-AP targets TM6SF2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag26527 Product name: Recombinant human TM6SF2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 289-377 aa of NM_001001524 Sequence: MFPGCSWLPDWALVFAGGIGQAQFSHMGASMHLRTPFTYRVPEDTWGCFFVCNLLYALGPHLLAYRCLQWPAFFHQPPPSDPLALHKKQH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transmembrane 6 superfamily member 2 |
| 别名 | KIAA1926, Transmembrane 6 superfamily member 2 |
| 计算分子量 | 43 kDa |
| 观测分子量 | 43 kDa |
| GenBank蛋白编号 | NM_001001524 |
| 基因名称 | TM6SF2 |
| Gene ID (NCBI) | 53345 |
| RRID | AB_3085983 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BZW4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TM6SF2 is mainly expressed in the liver, intestine, kidney, and in other tissues at low levels. In terms of subcellular localization, TM6SF2 is localized in the endoplasmic reticulum (ER), the ER-Golgi intermediate compartment, and Golgi of liver cells and enterocytes. The loss of TM6SF2 promotes the release of free fatty acids from IECs, leading to intestinal barrier dysfunction, gut microbiota dysbiosis and metabolite alterations (PMID: 29232562, 27013658). TM6SF2 encodes a protein predicted to have 7-10 transmembrane domains, containing 351 amino acids (39.5-kDa) with an estimated isoelectric point (pI) of 8.29 (PMID: 39779889).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for TM6SF2 antibody 27671-1-AP | Download protocol |
| WB protocol for TM6SF2 antibody 27671-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
