验证数据展示
经过测试的应用
| Positive IP detected in | Raji cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
17230-1-AP targets TLR9 in WB, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | rat |
| 免疫原 | TLR9 fusion protein Ag11071 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | toll-like receptor 9 |
| 别名 | CD289, toll like receptor 9, Toll-like receptor 9, UNQ5798/PRO19605 |
| 计算分子量 | 1032 aa, 116 kDa |
| 观测分子量 | 120 kDa, 60 kDa |
| GenBank蛋白编号 | BC032713 |
| 基因名称 | TLR9 |
| Gene ID (NCBI) | 54106 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NR96 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TLR9, also known as CD289, is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents and mediate the production of cytokines necessary for the development of effective immunity. TLR9 is a nucleotide-sensing TLR that is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides (PMID:14716310). Upon CpG stimulation, it induces B-cell proliferation, activation, survival, and antibody production (PMID:23857366). TLR9 acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion, and the inflammatory response (PMID:11564765, 17932028).
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for TLR9 antibody 17230-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |