验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, mouse liver tissue, RAW 264.7 cells, J774A.1 cells |
| Positive IHC detected in | human placenta tissue, mouse brain tissue, human liver cancer tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-Fro detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19811-1-AP targets TLR4 in WB, IHC, IF-Fro, IP, CoIP, ChIP, ELISA, Blocking assay, Microtiter plate binding assay, Western Blot applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-Fro, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, ChIP, ELISA, Blocking assay, Microtiter plate binding assay |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig, rabbit, chicken, zebrafish, hamster, goat |
| 免疫原 |
CatNo: Ag13841 Product name: Recombinant human TLR4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 653-839 aa of BC117422 Sequence: KFYFHLMLLAGCIKYGRGENIYDAFVIYSSQDEDWVRNELVKNLEEGVPPFQLCLHYRDFIPGVAIAANIIHEGFHKSRKVIVVVSQHFIQSRWCIFEYEIAQTWQFLSSRAGIIFIVLQKVEKTLLRQQVELYRLLSRNTYLEWEDSVLGRHIFWRRLRKALLDGKSWNPEGTVGTGCNWQEATSI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | toll-like receptor 4 |
| 别名 | ARMD10, CD284, hToll, TOLL, toll like receptor 4 |
| 计算分子量 | 839 aa, 96 kDa |
| 观测分子量 | 90-110 kDa |
| GenBank蛋白编号 | BC117422 |
| 基因名称 | TLR4 |
| Gene ID (NCBI) | 7099 |
| RRID | AB_10638446 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O00206 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TLR4, also named CD284, belongs to the Toll-like receptor family. TLR4 interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP, and TRAF6, leading to NF-kB activation, cytokine secretion, and the inflammatory response. Three alternatively spliced transcript variants that encode different protein isoforms have been described. The calculated molecular weights of the three TLR4 isoforms are 96, 91, and 73 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for TLR4 antibody 19811-1-AP | Download protocol |
| IHC protocol for TLR4 antibody 19811-1-AP | Download protocol |
| WB protocol for TLR4 antibody 19811-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Immunity Alarmin-loaded extracellular lipid droplets induce airway neutrophil infiltration during type 2 inflammation | ||
Immunity Intestinal dysbiosis exacerbates skin inflammation via microbial metabolite-driven Th2 cell differentiation. | ||
Adv Mater Biomimetic Immunosuppressive Exosomes that Inhibit Cytokine Storms Contribute to the Alleviation of Sepsis. | ||
Cell Mol Immunol MARCH2/3 target FcγRI for K27-linked polyubiquitination and degradation to restrict the inflammatory response. | ||
Bioact Mater Novel neutrophil extracellular trap-related mechanisms in diabetic wounds inspire a promising treatment strategy with hypoxia-challenged small extracellular vesicles | ||
Transl Neurodegener SSEA3 and CD105 positivity are associated with the treatment potency of human neural crest-derived nasal turbinate stem cells for Alzheimer's disease. |
