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验证数据展示

  • WB analysis using 83136-3-RR (same clone as 83136-3-PBS)

    WB analysis using 83136-3-RR (same clone as 83136-3-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 83136-3-RR (TLR3 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83136-3-PBS in a different storage buffer formulation.

  • IHC staining of human ovarian cancer using 83136-3-RR (same clone as 83136-3-PBS)

    IHC staining of human ovarian cancer using 83136-3-RR (same clone as 83136-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83136-3-RR (TLR3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83136-3-PBS in a different storage buffer formulation.

  • IHC staining of human ovarian cancer using 83136-3-RR (same clone as 83136-3-PBS)

    IHC staining of human ovarian cancer using 83136-3-RR (same clone as 83136-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83136-3-RR (TLR3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83136-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83136-3-RR

    Affinity and Kinetic Characterization of 83136-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83136-3-RR against Human TLR3 were performed. The affinity constant is below 1 pM.

TLR3/CD283 Recombinant antibody, PBS Only

TLR3/CD283 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 83136-3-PBS

产品说明书

CloneNo. 230445G4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

TLR3, 230445G4, CD283, toll like receptor 3, Toll-like receptor 3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83136-3-PBS targets TLR3/CD283 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原 TLR3/CD283 fusion protein Ag34044 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 toll-like receptor 3
别名 TLR3, 230445G4, CD283, toll like receptor 3, Toll-like receptor 3
计算分子量 904 aa, 104 kDa
观测分子量120 kDa, 68 kDa
GenBank蛋白编号BC094737
基因名称 TLR3
Gene ID (NCBI) 7098
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO15455
储存缓冲液 PBS only{{ptg:BufferTemp}}7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TLR3 belongs to the Toll-like receptor family which is important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR3 is a nucleotide-sensing TLR that is activated by double-stranded RNA. Upon recognition of dsRNA, TLR3 transmits signals via the adaptor protein TICAM-1/TRIF, leading to the induction of type I IFN, cytokine/chemokine production, and dendritic cell (DC) maturation (PMID: 18262679). Western detected full-length (∼120 kD) and cleaved (∼70 kDa) TLR3 (PMID: 28717003).