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  • Featured Product
  • KD/KO Validated

TLK2 Polyclonal antibody

TLK2 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 13979-1-AP

Print datasheet

Synonyms

PKU ALPHA, TLK2, tousled like kinase 2

验证数据展示

  • WB analysis of MCF-7 using 13979-1-AP

    WB analysis of MCF-7 using 13979-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 13979-1-AP (TLK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 13979-1-AP

    IHC staining of human breast cancer using 13979-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13979-1-AP (TLK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 13979-1-AP

    IHC staining of human breast cancer using 13979-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13979-1-AP (TLK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HeLa using 13979-1-AP

    WB analysis of HeLa using 13979-1-AP

    WB result of TLK2 antibody (13979-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-TLK2 transfected HeLa cells.

  • IF Staining of HeLa using 13979-1-AP

    IF Staining of HeLa using 13979-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13979-1-AP (TLK2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inMCF-7 cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13979-1-AP targets TLK2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen TLK2 fusion protein Ag5022 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tousled-like kinase 2
Synonyms PKU ALPHA, TLK2, tousled like kinase 2
Calculated Molecular Weight 772 aa, 88 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC044925
Gene Symbol TLK2
Gene ID (NCBI) 11011
RRIDAB_2203887
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86UE8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tousled-like kinases (TLKs) are a family of serine/threonine kinases located in the nucleus that promote S-phase chromatin assembly and mitotic chromosome segregation. There are two genes in the TLK family-TLK1 and TLK2 (PMID: 14561777). Mutation in TLK2 are associated with an elevated risk of breast cancer (PMID: 27489360). Tousled-like kinase 2 (TLK2) is a key factor in glioblastoma that modulates SRC signaling, thereby driving tumor malignancy. TLK2 is commonly upregulated in glioblastoma, and such upregulation was associated with poor patient outcomes. TLK2 overexpression induced cell growth, migration, invasion, and epithelialmesenchymal transition, and cell cycle arrest, while TLK2 knockdown had the opposite effect (PMID: 30207834). It can detect 3 isoforms of 88, 85 and 82 kDa.

实验方案

Product Specific Protocols
WB protocol for TLK2 antibody 13979-1-APDownload protocol
IHC protocol for TLK2 antibody 13979-1-APDownload protocol
IF protocol for TLK2 antibody 13979-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Gene Ther

Targeting TLK2 inhibits the progression of gastric cancer by reprogramming amino acid metabolism through the mTOR/ASNS axis

Authors - Mingliang Wang
  • KD Validated
View Article
humanIHC

Front Genet

Overexpression of tousled-like kinase 2 predicts poor prognosis in HBV-related hepatocellular carcinoma patients after radical resection

Authors - Bang Liu
View Article