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验证数据展示

  • WB analysis using 30959-1-AP (same clone as 30959-1-PBS)

    WB analysis using 30959-1-AP (same clone as 30959-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30959-1-AP (TLE4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30959-1-PBS in a different storage buffer formulation.

  • IP experiment of K-562 using 30959-1-AP (same clone as 30959-1-PBS)

    IP experiment of K-562 using 30959-1-AP (same clone as 30959-1-PBS)

    IP result of anti-TLE4 (IP:30959-1-AP, 4ug; Detection:30959-1-AP 1:800) with K-562 cells lysate 1720 ug. This data was developed using the same antibody clone with 30959-1-PBS in a different storage buffer formulation.

TLE4 Polyclonal antibody, PBS Only

TLE4 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 30959-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, Indirect ELISA

别名

GRG4, KIAA1261

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

30959-1-PBS targets TLE4 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag34006

Product name: Recombinant human TLE4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 279-408 aa of BC059405

Sequence: LDKTRLLKKDAPISPASIASSSSTPSSKSKELSLKRDMGKLSETRLSEDEQCTLGLQRWFCRLWFMNEKSTTPVSKSNTPTPRTDAPTPGSNSTPGLRPVPGKPPGVDPLASSLRTPMAVPCPYPTPFGI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila)
别名 GRG4, KIAA1261
计算分子量 84 kDa
观测分子量76-88 kDa
GenBank蛋白编号BC059405
基因名称 TLE4
Gene ID (NCBI) 7091
RRIDAB_3742381
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ04727
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TLE4 (Transducin-like enhancer protein 4), also known as GRG4. It is predicted to be located in the nucleus. Tle4 promotes acquisition of corticothalamic identity and blocks emergence of core characteristics of subcerebral/corticospinal projection neuron identity, including gene expression and connectivity. During the first post-natal week, when corticothalamic innervation is ongoing, Tle4 is required to stabilize corticothalamic neuron identity, limiting interference from differentiation programs of developmentally related neuron classes (PMID: 37561632). The molecular weight of TLE4 is 76 kDa.

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