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验证数据展示

  • WB analysis using 17135-1-AP

    WB analysis using 17135-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17135-1-AP (TKTL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of rat testis using 17135-1-AP

    IHC staining of rat testis using 17135-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17135-1-AP (TKTL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat testis using 17135-1-AP

    IHC staining of rat testis using 17135-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17135-1-AP (TKTL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 17135-1-AP

    IF Staining of HepG2 using 17135-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TKTL2 antibody (17135-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

TKTL2 Polyclonal antibody

TKTL2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 17135-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

EC:2.2.1.1, transketolase like 2, Transketolase like protein 2, Transketolase-like protein 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, mouse testis tissue
Positive IHC detected inrat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17135-1-AP targets TKTL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10884

Product name: Recombinant human TKTL2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 278-626 aa of BC028707

Sequence: IESQIQTNENLIPKSPVEDSPQISVTDIKMTSPHAYKVGDKIATQKTYGLALAKLGRANERVIVLSGDTMNSTFSEIFRKEHPERFIECIIAEQNMVSVALGCATRGRTIAFAGAFAAFFTRAFDQLRMGAISQANINLIGSHCGVSTGEDGVSQMALEDLAMFRSIPNCTVFYPSDAISTEHAIYLAANTKGMCFIRTSQPETAVIYTPQENFEIGQAKVVRHGVNDKVTVIGAGVTLHEALEAADHLSQQGISVRVIDPFTIKPLDAATIISSAKATGGRVITVEDHYREGGIGEAVCAAVSREPDILVHQLAVSGVPQRGKTSELLDMFGISTRHIIAAVTLTLMK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transketolase-like 2
别名 EC:2.2.1.1, transketolase like 2, Transketolase like protein 2, Transketolase-like protein 2
计算分子量 626 aa, 68 kDa
观测分子量68 kDa
GenBank蛋白编号BC028707
基因名称 TKTL2
Gene ID (NCBI) 84076
RRIDAB_3085516
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H0I9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transketolase-like 2 (TKTL2) plays an essential role in total transketolase activity and cell proliferation in cancer cells. TKTL2 is predicted to enable thiamine pyrophosphate binding activity and transketolase activity.

实验方案

Product Specific Protocols
IF protocol for TKTL2 antibody 17135-1-APDownload protocol
IHC protocol for TKTL2 antibody 17135-1-APDownload protocol
WB protocol for TKTL2 antibody 17135-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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