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验证数据展示

  • WB analysis using 87106-3-RR

    WB analysis using 87106-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87106-3-RR (TJAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 87106-3-RR

    IF Staining of A431 using 87106-3-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using TJAP1 antibody (87106-3-RR, Clone: 252301G9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity of 87106-3-RR

    Affinity of 87106-3-RR

    Biolayer interferometry (BLl) kinetic assays of 87106-3-RR against Human TJAP1 were performed. The affinity constant is 29.2 nM.

TJAP1 Recombinant monoclonal antibody

TJAP1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 87106-3-RR

产品说明书

CloneNo. 252301G9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

PILT, Tight junction-associated protein 1, TJAP 1, TJP4

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, MDA-MB-231 cells, HSC-T6 cells, 4T1 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87106-3-RR targets TJAP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12174

Product name: Recombinant human TJAP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-351 aa of BC064401

Sequence: MTSAAPAKKPYRKAPPEHRELRLEIPGSRLEQEEPLTDAERMKLLQEENEELRRRLASATRRTEALERELEIGQDCLELELGQSREELDKFKDKFRRLQNSYTASQRTNQELEDKLHTLIKKAEMDRKTLDWEIVELTNKLLDAKNTINKLEELNERYRLDCNLAVQLLKCNKSHFRNHKFADLPCELQDMVRKHLHSGQEAASPGPAPSLAPGAVVPTSVIARVLEKPESLLLNSAQSGSAGRPLAEDVFVHVDMSEGVPGDPASPPAPGSPTPQPNGECHSLGTARGSPEEELPLPAFEKLNPYPTPSPPHPLYPGRRVIEFSEDKVRIPRNSPLPNCTYATRQAISLS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tight junction associated protein 1 (peripheral)
别名 PILT, Tight junction-associated protein 1, TJAP 1, TJP4
计算分子量 547 aa, 61 kDa
观测分子量86-90 kDa
GenBank蛋白编号BC064401
基因名称 TJAP1
Gene ID (NCBI) 93643
RRIDAB_3745343
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ5JTD0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TJAP1, also named as PILT and TJP4, is colocalized at the tight junctions. TJAP1 has some forms with MW 60 kDa (native) and 86-90 kDa (modified).

实验方案

Product Specific Protocols
IF protocol for TJAP1 antibody 87106-3-RRDownload protocol
WB protocol for TJAP1 antibody 87106-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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