验证数据展示
经过测试的应用
| Positive WB detected in | fetal human brain tissue, BxPC-3 cells, HT-1080 cells, human heart tissue |
| Positive IHC detected in | human gliomas tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12326-1-AP targets TIMP-4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag2927 Product name: Recombinant human TIMP4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-224 aa of BC010553 Sequence: MPGSPRPAPSWVLLLRLLALLRPPGLGEACSCAPAHPQQHICHSALVIRAKISSEKVVPASADPADTEKMLRYEIKQIKMFKGFEKVKDVQYIYTPFDSSLCGVKLEANSQKQYLLTGQVLSDGKVFIHLCNYIEPWEDLSLVQRESLNHHYHLNCGCQITTCYTVPCTISAPNECLWTDWLLERKLYGYQAQHYVCMKHVDGTCSWYRGHLPLRKEFVDIVQP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | TIMP metallopeptidase inhibitor 4 |
| 别名 | TIMP4, Metalloproteinase inhibitor 4, TIMP 4 |
| 计算分子量 | 224 aa, 26 kDa |
| 观测分子量 | 26-30 kDa |
| GenBank蛋白编号 | BC010553 |
| 基因名称 | TIMP4 |
| Gene ID (NCBI) | 7079 |
| RRID | AB_10638303 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q99727 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TIMP-4 antibody 12326-1-AP | Download protocol |
| IHC protocol for TIMP-4 antibody 12326-1-AP | Download protocol |
| IF protocol for TIMP-4 antibody 12326-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Adv Res Novel function of macrophage migration inhibitory factor in regulating post-infarct inflammation and the therapeutic significance | ||
J Med Chem Discovery of a Novel Small-Molecule Inhibitor Disrupting TRBP-Dicer Interaction against Hepatocellular Carcinoma via the Modulation of microRNA Biogenesis. | ||
Respir Res An integrated machine learning model of transcriptomic genes in multi-center chronic obstructive pulmonary disease reveals the causal role of TIMP4 in airway epithelial cell | ||
Sci Rep WNTA5-mediated miR-374a-5p regulates vascular smooth muscle cell phenotype transformation and M1 macrophage polarization impacting intracranial aneurysm progression | ||
J Infect Dis Abundant Neutrophils-initiated Acute Myocardial Injury Following Coxsackievirus A6 Infection | ||
