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TIMM8A Polyclonal antibody

TIMM8A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11179-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

TIM8A, TIM8, MTS, DFN1, DDP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11179-1-AP targets TIMM8A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 TIMM8A fusion protein Ag1661 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 translocase of inner mitochondrial membrane 8 homolog A (yeast)
别名 TIM8A, TIM8, MTS, DFN1, DDP1
计算分子量 11 kDa
观测分子量 11 kDa
GenBank蛋白编号BC015093
基因名称 TIMM8A
Gene ID (NCBI) 1678
RRIDAB_2204687
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60220
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TIMM8A antibody 11179-1-APDownload protocol
IHC protocol for TIMM8A antibody 11179-1-APDownload protocol
IF protocol for TIMM8A antibody 11179-1-APDownload protocol
IP protocol for TIMM8A antibody 11179-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Nature

Stress response silencing by an E3 ligase mutated in neurodegeneration

Authors - Diane L Haakonsen
humanWB

Cell Death Dis

CHCHD2 rescues the mitochondrial dysfunction in iPSC-derived neurons from patient with Mohr-Tranebjaerg syndrome

Authors - Yihua Huang
  • KD Validated
humanWB

Elife

Function of hTim8a in complex IV assembly in neuronal cells provides insight into pathomechanism underlying Mohr-Tranebjærg syndrome.

Authors - Yilin Kang
  • KO Validated
WB

Cell Death Dis

Haploinsufficiency in the mitochondrial protein CHCHD4 reduces brain injury in a mouse model of neonatal hypoxia-ischemia.

Authors - Yanyan Sun
humanWB

Mol Genet Genomic Med

Functional analysis of a novel mutation in the TIMM8A gene that causes deafness-dystonia-optic neuronopathy syndrome.

Authors - Addison Neighbors
mouseIF

Genes Dis

Cdc42 deletion yielded enamel defects by disrupting mitochondria and producing reactive oxygen species in dental epithelium

Authors - Jinxuan Zheng
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