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  • Featured Product
  • KD/KO Validated

TIMM8A Polyclonal antibody

TIMM8A Polyclonal Antibody for IF/ICC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 11179-1-AP

Print datasheet

Synonyms

DDP, DDP1, Deafness dystonia protein 1, DFN1, MTS, TIM8, TIM8A, TIMM8A

验证数据展示

  • WB analysis using 11179-1-AP

    WB analysis using 11179-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11179-1-AP (TIMM8A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 11179-1-AP

    IF Staining of HeLa using 11179-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11179-1-AP (TIMM8A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IP experiment of mouse testis using 11179-1-AP

    IP experiment of mouse testis using 11179-1-AP

    IP result of anti-TIMM8A (IP:11179-1-AP, 4ug; Detection:11179-1-AP 1:500) with mouse testis tissue lysate 4000ug.

经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11179-1-AP targets TIMM8A in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen TIMM8A fusion protein Ag1661 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name translocase of inner mitochondrial membrane 8 homolog A (yeast)
Synonyms DDP, DDP1, Deafness dystonia protein 1, DFN1, MTS, TIM8, TIM8A, TIMM8A
Calculated Molecular Weight 11 kDa
Observed Molecular Weight 11 kDa
GenBank Accession NumberBC015093
Gene Symbol TIMM8A
Gene ID (NCBI) 1678
RRIDAB_2204687
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60220
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TIMM8A antibody 11179-1-APDownload protocol
IF protocol for TIMM8A antibody 11179-1-APDownload protocol
IP protocol for TIMM8A antibody 11179-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Elife

Function of hTim8a in complex IV assembly in neuronal cells provides insight into pathomechanism underlying Mohr-Tranebjærg syndrome.

Authors - Yilin Kang
  • KO Validated
View Article
WB

Cell Death Dis

Haploinsufficiency in the mitochondrial protein CHCHD4 reduces brain injury in a mouse model of neonatal hypoxia-ischemia.

Authors - Yanyan Sun
View Article
humanWB

Mol Genet Genomic Med

Functional analysis of a novel mutation in the TIMM8A gene that causes deafness-dystonia-optic neuronopathy syndrome.

Authors - Addison Neighbors
View Article