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验证数据展示

  • WB analysis using 85134-5-RR (same clone as 85134-5-PBS)

    WB analysis using 85134-5-RR (same clone as 85134-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85134-5-RR (TIMM44 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85134-5-PBS in a different storage buffer formulation.

  • WB analysis of Neuro-2a using 85134-5-RR (same clone as 85134-5-PBS)

    WB analysis of Neuro-2a using 85134-5-RR (same clone as 85134-5-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 85134-5-RR (TIMM44 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85134-5-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 85134-5-RR (same clone as 85134-5-PBS)

    IF Staining of HepG2 using 85134-5-RR (same clone as 85134-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TIMM44 antibody (85134-5-RR, Clone: 242774A4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85134-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85134-5-RR

    Affinity and Kinetic Characterization of 85134-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85134-5-RR against Human TIMM44 were performed. The affinity constant is below 1 pM.

TIMM44 Recombinant antibody, PBS Only

TIMM44 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85134-5-PBS

产品说明书

CloneNo. 242774A4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

TIMM 44, TIM44, MIMT44, 242774A4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

85134-5-PBS targets TIMM44 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 TIMM44 fusion protein Ag4834 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 translocase of inner mitochondrial membrane 44 homolog (yeast)
别名 TIMM 44, TIM44, MIMT44, 242774A4
计算分子量 452 aa, 51 kDa
观测分子量45 kDa
GenBank蛋白编号BC033628
基因名称 TIMM44
Gene ID (NCBI) 10469
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO43615
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Translocase of inner mitochondrial membrane 44 (TIMM44), also known as MIMT44 and TIM44, belongs to the Tim44 family. TIMM44 is a mitochondrial protein located at the inner mitochondrial membrane, which is crucial for the integrity and function of mitochondria. In an ATP-dependent manner, TIMM44 anchors mitochondrial heat shock protein 70 to the translocase of the mitochondrial inner membrane 23 (TIMM23) complex. TIMM44 is essential for mitochondrial pre-protein import into the mitochondrial matrix. TIMM44 is vital for the integrity and function of mitochondria. TIMM44 silencing disrupted mitochondrial functions in endothelial cells, causing mitochondrial protein input arrest, ATP reduction, ROS production, and mitochondrial depolarization, and leading to apoptosis activation (PMID: 36438483, PMID: 37147302, PMID: 38467612). The observed molecular weight of TIMM44 is 45 kDa, indicating a mature form of TIMM44 (PMID: 33891006).