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验证数据展示

  • WB analysis using 66149-1-Ig (same clone as 66149-1-PBS)

    WB analysis using 66149-1-Ig (same clone as 66149-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66149-1-Ig (TIMM44 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66149-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66149-1-Ig (same clone as 66149-1-PBS)

    IHC staining of human liver using 66149-1-Ig (same clone as 66149-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66149-1-Ig (TIMM44 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66149-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66149-1-Ig (same clone as 66149-1-PBS)

    WB analysis of HeLa using 66149-1-Ig (same clone as 66149-1-PBS)

    WB result of TIMM44 antibody (66149-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TIMM44 transfected HeLa cells. This data was developed using the same antibody clone with 66149-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66149-1-Ig (same clone as 66149-1-PBS)

    IF Staining of HepG2 using 66149-1-Ig (same clone as 66149-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TIMM44 antibody (66149-1-Ig, Clone: 4G7D4 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66149-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TIMM44 Monoclonal antibody, PBS Only

TIMM44 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66149-1-PBS

产品说明书

CloneNo. 4G7D4

宿主/亚型

Mouse / IgG2c

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

4G7D4, MIMT44, TIM44, TIMM 44

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66149-1-PBS targets TIMM44 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原

CatNo: Ag21389

Product name: Recombinant human TIMM44 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 153-452 aa of BC033628

Sequence: AKTAKQSAESVSKGGEKLGRTAAFRALSQGVESVKKEIDDSVLGQTGPYRRPQRLRKRTEFAGDKFKEEKVFEPNEEALGVVLHKDSKWYQQWKDFKENNVVFNRFFEMKMKYDESDNAFIRASRALTDKVTDLLGGLFSKTEMSEVLTEILRVDPAFDKDRFLKQCENDIIPNVLEAMISGELDILKDWCYEATYSQLAHPIQQAKALGLQFHSRILDIDNVDLAMGKMMEQGPVLIITFQAQLVMVVRNPKGEVVEGDPDKVLRMLYVWALCRDQDELNPYAAWRLLDISASSTEQIL

种属同源性预测
宿主/亚型 Mouse / IgG2c
抗体类别 Monoclonal
产品类型 Antibody
全称 translocase of inner mitochondrial membrane 44 homolog (yeast)
别名 4G7D4, MIMT44, TIM44, TIMM 44
计算分子量 452 aa, 51 kDa
观测分子量 45 kDa
GenBank蛋白编号BC033628
基因名称 TIMM44
Gene ID (NCBI) 10469
RRIDAB_2881545
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO43615
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Translocase of inner mitochondrial membrane 44 (TIMM44), also known as MIMT44 and TIM44, belongs to the Tim44 family. TIMM44 is a mitochondrial protein located at the inner mitochondrial membrane, which is crucial for the integrity and function of mitochondria. In an ATP-dependent manner, TIMM44 anchors mitochondrial heat shock protein 70 to the translocase of the mitochondrial inner membrane 23 (TIMM23) complex. TIMM44 is essential for mitochondrial pre-protein import into the mitochondrial matrix. TIMM44 is vital for the integrity and function of mitochondria. TIMM44 silencing disrupted mitochondrial functions in endothelial cells, causing mitochondrial protein input arrest, ATP reduction, ROS production, and mitochondrial depolarization, and leading to apoptosis activation (PMID: 36438483, PMID: 37147302, PMID: 38467612). The observed molecular weight of TIMM44 is 45 kDa, indicating a mature form of TIMM44 (PMID: 33891006).

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