验证数据展示
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IHC detected in | mouse colon tissue, mouse small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
产品信息
22136-1-AP targets TIGAR in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | TIGAR fusion protein Ag17532 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 12 open reading frame 5 |
| 别名 | C12orf5, TP53-induced glycolysis regulatory phosphatase, TP53-induced glycolysis and apoptosis regulator, Fructose-2,6-bisphosphatase TIGAR, EC:3.1.3.46 |
| 计算分子量 | 270 aa, 30 kDa |
| 观测分子量 | 30 kDa |
| GenBank蛋白编号 | BC012340 |
| 基因名称 | TIGAR |
| Gene ID (NCBI) | 57103 |
| RRID | AB_2879003 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NQ88 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The TP53-induced glycolysis and apoptosis regulator (TIGAR), first reported in 2006, is a downstream target gene of p53 and an important factor involved in glycolysis and apoptosis . It is indispensable in metabolism and is involved in metabolic syndrome, including hyperglycemia, insulin resistance, alcoholic fatty liver and tissue ischemia. TIGAR is highly expressed in many cancer cells to promote cell survival.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TIGAR antibody 22136-1-AP | Download protocol |
| IHC protocol for TIGAR antibody 22136-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Free Radic Biol Med Oxidative stress-induced alterations in retinal glucose metabolism in Retinitis Pigmentosa. | ||
Neurobiol Dis Maternal Herpesviridae infection during pregnancy alters midbrain dopaminergic signatures in adult offspring. | ||
Cell Death Dis MiR-652-5p elevated glycolysis level by targeting TIGAR in T-cell acute lymphoblastic leukemia. | ||
Biochem Biophys Res Commun Cordycepin exhibits anti-fatigue effect via activating TIGAR/SIRT1/PGC-1α signaling pathway | ||
Phytother Res Dual targeting of wild-type p53 and gut microbiota by Magnolol represses key metabolic process and kills CRC cells | ||
Cancer Sci Impact of TP53-induced glycolysis and apoptosis regulator on malignant activity and resistance to ferroptosis in intrahepatic cholangiocarcinoma
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