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Tie1 Polyclonal antibody

Tie1 Polyclonal Antibody for FC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, WB,ELISA

Conjugate

Unconjugated

Cat No : 19329-1-AP

Print datasheet

Synonyms

JTK14, TIE, TIE1

验证数据展示

  • WB analysis of K-562 using 19329-1-AP

    WB analysis of K-562 using 19329-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 19329-1-AP (TIE1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • FC experiment of HUVEC using 19329-1-AP

    FC experiment of HUVEC using 19329-1-AP

    1X10^6 HUVEC cells were stained with 0.2ug TIE1 antibody (19329-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

经过测试的应用

Positive WB detected inK-562 cells, MCF-7 cells, NIH/3T3 cells
Positive FC detected inHUVEC cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 19329-1-AP is Tie1 Fusion Protein expressed in E. coli.

Tested Applications FC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen Tie1 fusion protein Ag4728 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tyrosine kinase with immunoglobulin-like and EGF-like domains 1
Synonyms JTK14, TIE, TIE1
Calculated Molecular Weight 1138 aa, 125 kDa
Observed Molecular Weight 154 kDa
GenBank Accession NumberBC038239
Gene Symbol TIE1
Gene ID (NCBI) 7075
RRIDAB_10863655
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35590
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Tie1 antibody 19329-1-APDownload protocol
FC protocol for Tie1 antibody 19329-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols