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TICAM2 Polyclonal antibody

TICAM2 Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 19524-1-AP

Print datasheet

Synonyms

TICAM 2, TICAM2, TIRAP3, TIRP, TRAM, TRIF related adapter molecule

验证数据展示

  • WB analysis of PC-3 using 19524-1-AP

    WB analysis of PC-3 using 19524-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 19524-1-AP (TICAM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 19524-1-AP

    IHC staining of human testis using 19524-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19524-1-AP (TICAM2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 19524-1-AP

    IHC staining of human testis using 19524-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19524-1-AP (TICAM2 Antibody) at dilution of 1:50 (under 40x lens).

  • FC experiment of HeLa using 19524-1-AP

    FC experiment of HeLa using 19524-1-AP

    1X10^6 HeLa cells were stained with 0.2ug TICAM2 antibody (19524-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

经过测试的应用

Positive WB detected inPC-3 cells, HeLa cells, human testis tissue
Positive IHC detected inhuman testis tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19524-1-AP targets TICAM2 in WB, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IHC, WB,ELISA
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name toll-like receptor adaptor molecule 2
Synonyms TICAM 2, TICAM2, TIRAP3, TIRP, TRAM, TRIF related adapter molecule
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberNM_021649
Gene Symbol TICAM2
Gene ID (NCBI) 353376
RRIDAB_10791887
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86XR7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TICAM2, also named as TIRAP3, TIRP and TRAM, is involved in IL1-triggered NF-kappa-B activation, functioning upstream of IRAK1, IRAK2, TRAF6, and IKBKB. Physically bridges TLR4 and TICAM1 and functionally transmits LPS-TRL4 signal to TICAM1. TICAM2 is involved in LPS-TLR4 signaling to regulate the MYD88-independent pathway during the innate immune response to LPS.In WB, the MW of TICAM2 is about 27-32kd.

实验方案

Product Specific Protocols
WB protocol for TICAM2 antibody 19524-1-APDownload protocol
IHC protocol for TICAM2 antibody 19524-1-APDownload protocol
FC protocol for TICAM2 antibody 19524-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols