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验证数据展示

  • WB analysis using 19524-1-AP

    WB analysis using 19524-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 19524-1-AP (TICAM2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 19524-1-AP

    IHC staining of mouse testis using 19524-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19524-1-AP (TICAM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 19524-1-AP

    IHC staining of mouse brain using 19524-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19524-1-AP (TICAM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TICAM2 Polyclonal antibody

TICAM2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19524-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

MyD88-4, Putative NF-kappa-B-activating protein 502, TICAM 2, TICAM-2, TIR domain-containing adapter molecule 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, HeLa cells, human testis tissue, PC-3 cells
Positive IHC detected inmouse testis tissue, human prostate hyperplasia tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19524-1-AP targets TICAM2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 toll-like receptor adaptor molecule 2
别名 MyD88-4, Putative NF-kappa-B-activating protein 502, TICAM 2, TICAM-2, TIR domain-containing adapter molecule 2
计算分子量 27 kDa
观测分子量 32 kDa
GenBank蛋白编号NM_021649
基因名称 TICAM2
Gene ID (NCBI) 353376
RRIDAB_10791887
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86XR7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TICAM2, also named TIRAP3, TIRP, and TRAM, is involved in IL1-triggered NF-kappa-B activation, functioning upstream of IRAK1, IRAK2, TRAF6, and IKBKB. Physically bridges TLR4 and TICAM1 and functionally transmits LPS-TRL4 signal to TICAM1. TICAM2 is involved in LPS-TLR4 signaling to regulate the MYD88-independent pathway during the innate immune response to LPS.In WB, the MW of TICAM2 is about 27-32 kDa.

实验方案

Product Specific Protocols
WB protocol for TICAM2 antibody 19524-1-APDownload protocol
IHC protocol for TICAM2 antibody 19524-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols