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验证数据展示

  • WB analysis of PC-3 using 20328-1-AP

    WB analysis of PC-3 using 20328-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 20328-1-AP (TIAF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin using 20328-1-AP

    IHC staining of human skin using 20328-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 20328-1-AP (TIAF1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human skin using 20328-1-AP

    IHC staining of human skin using 20328-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 20328-1-AP (TIAF1 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of PC-3 using 20328-1-AP

    IF Staining of PC-3 using 20328-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 20328-1-AP (TIAF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

TIAF1 Polyclonal antibody

TIAF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20328-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

MAJN, MYO18A, MYSPDZ, SPR210, TIAF1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inPC-3 cells
Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20328-1-AP targets TIAF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag14023

Product name: Recombinant human TIAF1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-115 aa of BC131561

Sequence: MSSPSSPFREQSFLCAAGDAGEESRVQVLKNEVRRGSPVLLGWVEQAYADKCVCGPSAPPAPTPPSLSQRVMCNDLFKVNPFQLQQFRADPSTASLLLCPGGLDHKLNLRGKAWG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TGFB1-induced anti-apoptotic factor 1
别名 MAJN, MYO18A, MYSPDZ, SPR210, TIAF1
计算分子量 115 aa, 12 kDa
观测分子量 12 kDa
GenBank蛋白编号BC131561
基因名称 TIAF1
Gene ID (NCBI) 9220
RRIDAB_10858233
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95411
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TIAF1 antibody 20328-1-APDownload protocol
IHC protocol for TIAF1 antibody 20328-1-APDownload protocol
IF protocol for TIAF1 antibody 20328-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
HumanIHC

Oncogenesis

HOXC8 promotes proliferation and migration through transcriptional up-regulation of TGFβ1 in non-small cell lung cancer.

Authors - Houli Liu
View Article
humanWB

Mol Carcinog

Upregulation of MGP by HOXC8 promotes the proliferation, migration, and EMT processes of triple-negative breast cancer.

Authors - Chen Gong
View Article