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THUMPD3 Polyclonal antibody

THUMPD3 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 19807-1-AP

Print datasheet

Synonyms

THUMP domain containing 3, THUMPD3

验证数据展示

  • WB analysis of HeLa using 19807-1-AP

    WB analysis of HeLa using 19807-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19807-1-AP (THUMPD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human hepatocirrhosis using 19807-1-AP

    IHC staining of human hepatocirrhosis using 19807-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19807-1-AP (THUMPD3 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human hepatocirrhosis using 19807-1-AP

    IHC staining of human hepatocirrhosis using 19807-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19807-1-AP (THUMPD3 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HeLa using 19807-1-AP

    IF Staining of HeLa using 19807-1-AP

    Immunofluorescent analysis of Hela cells, using THUMPD3 antibody 19807-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, mouse kidney tissue
Positive IHC detected inhuman hepatocirrhosis tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells, HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 19807-1-AP is THUMPD3 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen THUMPD3 fusion protein Ag13833 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name THUMP domain containing 3
Synonyms THUMP domain containing 3, THUMPD3
Calculated Molecular Weight 507 aa, 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC001622
Gene Symbol THUMPD3
Gene ID (NCBI) 25917
RRIDAB_10644140
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BV44
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for THUMPD3 antibody 19807-1-APDownload protocol
IHC protocol for THUMPD3 antibody 19807-1-APDownload protocol
IF protocol for THUMPD3 antibody 19807-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols