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验证数据展示

  • WB analysis using 19807-1-AP

    WB analysis using 19807-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19807-1-AP (THUMPD3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human hepatocirrhosis using 19807-1-AP

    IHC staining of human hepatocirrhosis using 19807-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19807-1-AP (THUMPD3 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human hepatocirrhosis using 19807-1-AP

    IHC staining of human hepatocirrhosis using 19807-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19807-1-AP (THUMPD3 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HeLa using 19807-1-AP

    IF Staining of HeLa using 19807-1-AP

    Immunofluorescent analysis of Hela cells, using THUMPD3 antibody 19807-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

THUMPD3 Polyclonal antibody

THUMPD3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 19807-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

EC:2.1.1.256, THUMP domain containing 3, THUMP domain-containing protein 3, tRNA (guanine(6)-N2)-methyltransferase THUMP3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, mouse kidney tissue, L02 cells
Positive IHC detected inhuman hepatocirrhosis tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells, HepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19807-1-AP targets THUMPD3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag13833

Product name: Recombinant human THUMPD3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 161-507 aa of BC001622

Sequence: GQEVKIDQRNVKKEFTSHALDSHILDYYENPAIKEDVSTLIGDDLASCKDETDESSKEETEPQVLKFRVTCNRAGEKHCFTSNEAARDFGGAVQDYFKWKADMTNFDVEVLLNIHDNEVIVGIALTEESLHRRNITHFGPTTLRSTLAYGMLRLCDPLPYDIIVDPMCGTGAIPIEGATEWSDCFHIAGDNNPLAVNRAANNIASLLTKSQIKEGKPSWGLPIDAVQWDICNLPLRTGSVDIIVTDLPFGKRMGSKKRNWNLYPACLREMSRVCTPTTGRAVLLTQDTKCFTKALSGMRHVWRKVDTVWVNVGGLRAAVYVLIRTPQAFVHPSEQDGERGTLWQCKE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 THUMP domain containing 3
别名 EC:2.1.1.256, THUMP domain containing 3, THUMP domain-containing protein 3, tRNA (guanine(6)-N2)-methyltransferase THUMP3
计算分子量 507 aa, 57 kDa
观测分子量 57 kDa
GenBank蛋白编号BC001622
基因名称 THUMPD3
Gene ID (NCBI) 25917
RRIDAB_10644140
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BV44
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

THUMPD3, or THUMP domain containing 3, is a protein-coding gene that plays a significant role in tRNA methylation, a post-transcriptional modification that affects tRNA biology and is closely associated with human diseases. THUMPD3 is responsible for catalyzing the formation of N2-methylguanosine (m2G) at position 6 in a broad range of tRNA substrates containing the characteristic 3'-CCA terminus of mature tRNAs.

实验方案

Product Specific Protocols
IF protocol for THUMPD3 antibody 19807-1-APDownload protocol
IHC protocol for THUMPD3 antibody 19807-1-APDownload protocol
WB protocol for THUMPD3 antibody 19807-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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