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验证数据展示

  • IHC staining of human breast cancer using 14921-1-AP

    IHC staining of human breast cancer using 14921-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14921-1-AP (THUMPD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with THUMPD1 antibody included
  • WB analysis using 14921-1-AP

    WB analysis using 14921-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14921-1-AP (THUMPD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 14921-1-AP

    IF Staining of MCF-7 using 14921-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using THUMPD1 antibody (14921-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

THUMPD1 Polyclonal antibody

THUMPD1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14921-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

THUMP domain-containing protein 1, THUMPD 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, HeLa cells, MCF-7 cells, T-47D cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy THUMPD1 Ready-To-Use IHC Kit. THUMPD1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14921-1-AP targets THUMPD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag6739

Product name: Recombinant human THUMPD1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-353 aa of BC000448

Sequence: MAAPAQQTTQPGGGKRKGKAQYVLAKRARRCDAGGPRQLEPGLQGILITCNMNERKCVEEAYSLLNEYGDDMYGPEKFTDKDQQPSGSEGEDDDAEAALKKEVGDIKASTEMRLRRFQSVESGANNVVFIRTLGIEPEKLVHHILQDMYKTKKKKTRVILRMLPISGTCKAFLEDMKKYAETFLEPWFKAPNKGTFQIVYKSRNNSHVNREEVIRELAGIVCTLNSENKVDLTNPQYTVVVEIIKAVCCLSVVKDYMLFRKYNLQEVVKSPKDPSQLNSKQGNGKEAKLESADKSDQNNTAEGKNNQQVPENTEELGQTKPTSNPQVVNEGGAKPELASQATEGSKSNENDFS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 THUMP domain containing 1
别名 THUMP domain-containing protein 1, THUMPD 1
计算分子量 39 kDa
观测分子量 49 kDa
GenBank蛋白编号BC000448
基因名称 THUMPD1
Gene ID (NCBI) 55623
RRIDAB_2271785
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NXG2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

THUMPD1, a THUMP domain-containing RNA-binding protein, partners with NAT10 to mediate N4-acetylcytidine (ac4C) modification, enhancing RNA stability, export, and translation. Dysregulated THUMPD1 promotes proliferation, migration, invasion, and EMT.

实验方案

Product Specific Protocols
WB protocol for THUMPD1 antibody 14921-1-APDownload protocol
IHC protocol for THUMPD1 antibody 14921-1-APDownload protocol
IF protocol for THUMPD1 antibody 14921-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Differ

Lactylation of NAT10 promotes N4-acetylcytidine modification on tRNASer-CGA-1-1 to boost oncogenic DNA virus KSHV reactivation

Authors - Qin Yan
View Article
humanIF

Biosci Rep

Pan-cancer analysis of N4-acetylcytidine adaptor THUMPD1 as a predictor for prognosis and immunotherapy.

Authors - Kuangxun Li
View Article
humanIHC

iScience

RNA modification-related genes illuminate prognostic signature and mechanism in esophageal squamous cell carcinoma

Authors - Hui Han
View Article