验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, LNCaP cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human ovary tumor tissue, human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy THRAP3 Ready-To-Use IHC Kit. THRAP3 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19744-1-AP targets THRAP3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, pig |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | thyroid hormone receptor associated protein 3 |
| 别名 | TRAP150, Thyroid hormone receptor-associated protein complex 150 kDa component, Thyroid hormone receptor-associated protein 3, BCLAF2, BCLAF1 and THRAP3 family member 2 |
| 计算分子量 | 109 kDa |
| 观测分子量 | 109-130 kDa |
| GenBank蛋白编号 | NM_005119 |
| 基因名称 | THRAP3 |
| Gene ID (NCBI) | 9967 |
| RRID | AB_11232035 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y2W1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
THRAP3, also named as TRAP150, plays a role in transcriptional coactivation. The antibody recognizes the C-term of THRAP3.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for THRAP3 antibody 19744-1-AP | Download protocol |
| IHC protocol for THRAP3 antibody 19744-1-AP | Download protocol |
| IF protocol for THRAP3 antibody 19744-1-AP | Download protocol |
| IP protocol for THRAP3 antibody 19744-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res The N6-methyladenosine RNA-binding protein YTHDF1 modulates the translation of TRAF6 to mediate the intestinal immune response. | ||
J Cell Sci Novel stress granules-like structures are induced via a paracrine mechanism during viral infection. | ||
Cancer Res NAT10 drives cisplatin chemoresistance by enhancing ac4C-associated DNA repair in bladder cancer | ||
Mol Cell Proteomics Deciphering the clinical significance and kinase functions of GSK3α in colon cancer by proteomics and phosphoproteomics | ||
Commun Biol NLRP7 maintains the genomic stability during early human embryogenesis via mediating alternative splicing |
